ArticleJournal of materials science. Materials in medicine2011
Preparation of chitosan films using different neutralizing solutions to improve endothelial cell compatibility.
Article in Journal of materials science. Materials in medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Influence of Cross-Linking Agents on the Structure and Stability of Chitosan and Carboxymethyl Chitosan Thin Films.Molecules (Basel, Switzerland) · 2026Article
- Freeze-Dried Chitosan Scaffolds Containing Grape Seed Oil for Wound Healing Applications.ACS omega · 2025Article
- Environmental Influence on Degradation of Chitosan Bioplastics.ACS omega · 2025Article
- Water vapor transport properties of bio-based multilayer materials determined by original and complementary methods.Scientific reports · 2024Article
- Risedronate-loaded aerogel scaffolds for bone regeneration.Drug delivery · 2023Article
- Rheological and Film-Forming Properties of Chitosan Composites.International journal of molecular sciences · 2022Article
- Regulating the Physicochemical Properties of Chitosan Films through Concentration and Neutralization.Foods (Basel, Switzerland) · 2022Article
- Physicochemical Properties and Cell Viability of Shrimp Chitosan Films as Affected by Film Casting Solvents. I-Potential Use as Wound Dressing.Materials (Basel, Switzerland) · 2020Article
- Ionic Conductivity and Structure of Chitosan Films Modified with Lactic Acid-Choline Chloride NADES.Polymers · 2020Article
- Article
- Endothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.Journal of translational medicine · 2017Article
- Blow-spun chitosan/PEG/PLGA nanofibers as a novel tissue engineering scaffold with antibacterial properties.Journal of materials science. Materials in medicine · 2016Article
- Co-fabrication of chitosan and epoxy photoresist to form microwell arrays with permeable hydrogel bottoms.Biomaterials · 2016Article
- Development and Characterization of Novel Films Based on Sulfonamide-Chitosan Derivatives for Potential Wound Dressing.International journal of molecular sciences · 2015Article
- Fabrication and evaluation of electrospun collagen/poly(N-isopropyl acrylamide)/chitosan mat as blood-contacting biomaterials for drug delivery.Journal of materials science. Materials in medicine · 2012Article
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4 authors.
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Abstract
The development of chitosan-based constructs for application in large-size defects or highly vascularized tissues is still a challenging issue. The poor endothelial cell compatibility of chitosan hinders the colonization of vascular endothelial cells in the chitosan-based constructs, and retards the establishment of a functional microvascular network following implantation. The aim of the present study is to prepare chitosan films with different neutralization methods to improve their endothelial cell compatibility. Chitosan salt films were neutralized with either sodium hydroxide (NaOH) aqueous solution, NaOH ethanol solution, or ethanol solution without NaOH. The physicochemical properties and endothelial cell compatibility of the chitosan films were investigated. Results indicated that neutralization with different solutions affected the surface chemistry, swelling ratio, crystalline conformation, nanotopography, and mechanical properties of the chitosan films. The NaOH ethanol solution-neutralized chitosan film (Chi-NaOH/EtOH film) displayed a nanofiber-dominant surface, while the NaOH aqueous solution-neutralized film (Chi-NaOH/H(2)O film) and the ethanol solution-neutralized film (Chi-EtOH film) displayed nanoparticle-dominant surfaces. Moreover, the Chi-NaOH/EtOH films exhibited a higher stiffness as compared to the Chi-NaOH/H(2)O and Chi-EtOH films. Endothelial cell compatibility of the chitosan films was evaluated with a human microvascular endothelial cell line, HMEC-1. Compared with the Chi-NaOH/H(2)O and Chi-EtOH films, HMECs cultured on the Chi-NaOH/EtOH films fully spread and exhibited significantly higher levels of adhesion and proliferation, with retention of the endothelial phenotype and function. Our findings suggest that the surface nanotopography and mechanical properties contribute to determining the endothelial cell compatibility of chitosan films. The nature of the neutralizing solutions can affect the physicochemical properties and endothelial cell compatibility of chitosan films. Therefore, selection of suitable neutralization methods is highly important for the application of chitosan in tissue engineering.
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