ArticleJournal of molecular biology2011
Crystal structure of the ligand binding domain of netrin G2.
Article in Journal of molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Membrane trafficking of synaptic adhesion molecules.The Journal of physiology · 2025Review
- "Netrin-slaying nanoparticles: A new frontier in cancer therapy".BBA advances · 2025Review
- Identifying the molecular basis of Laminin N-terminal domain CaBiophysical journal · 2024Article
- Article
- Building branched tissue structures: from single cell guidance to coordinated construction.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Crystallographic analysis of the laminin β2 short arm reveals how the LF domain is inserted into a regular array of LE domains.Matrix biology : journal of the International Society for Matrix Biology · 2017Article
- Diversification of behavior and postsynaptic properties by netrin-G presynaptic adhesion family proteins.Molecular brain · 2016Article
- Control of neural circuit formation by leucine-rich repeat proteins.Trends in neurosciences · 2014Review
- The crystal structure of netrin-1 in complex with DCC reveals the bifunctionality of netrin-1 as a guidance cue.Neuron · 2014Article
- Neural migration. Structures of netrin-1 bound to two receptors provide insight into its axon guidance mechanism.Science (New York, N.Y.) · 2014Article
- Anomalous diffraction in crystallographic phase evaluation.Quarterly reviews of biophysics · 2014Review
- Robust structural analysis of native biological macromolecules from multi-crystal anomalous diffraction data.Acta crystallographica. Section D, Biological crystallography · 2013Article
- Structures from anomalous diffraction of native biological macromolecules.Science (New York, N.Y.) · 2012Article
- Crystal structures of the network-forming short-arm tips of the laminin β1 and γ1 chains.PloS one · 2012Article
- Laminins in basement membrane assembly.Cell adhesion & migrationReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Netrin G proteins represent a small family of synaptic cell adhesion molecules related to netrins and to the polymerization domains of laminins. Two netrin G proteins are encoded in vertebrate genomes, netrins G1 and G2, which are known to bind the leucine-rich repeat proteins netrin G ligand (NGL)-1 and NGL-2, respectively. Netrin G proteins share a common multi-domain architecture comprising a laminin N-terminal (LN) domain followed by three laminin epidermal growth factor-like (LE) domains and a C' region containing a glycosylphosphatidylinositol anchor. Here, we use deletion analysis to show that the LN domain region of netrin Gs contains the binding site for NGLs to which they bind with 1:1 stoichiometry and sub-micromolar affinity. Netrin Gs are alternatively spliced in their LE domain regions, but the binding region, the LN domain, is identical in all splice forms. We determined the crystal structure for a fragment comprising the LN domain and domain LE1 of netrin G2 by sulfur single-wavelength anomalous diffraction phasing and refined it to 1.8 Å resolution. The structure reveals an overall architecture similar to that of laminin α chain LN domains but includes significant differences including a Ca(2+) binding site in the LN domain. These results reveal the minimal binding unit for interaction of netrin Gs with NGLs, define structural features specific to netrin Gs, and suggest that netrin G alternative splicing is not involved in NGL recognition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.