ArticleBioorganic & medicinal chemistry letters2011
Synthetase polyspecificity as a tool to modulate protein function.
Article in Bioorganic & medicinal chemistry letters, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Unnatural Amino Acid and Emerging Chemistry Approaches to Map RNA-Protein Interactions.Angewandte Chemie (International ed. in English) · 2026Review
- 3-iodotyrosine fluorescent proteins for improved expansion microscopy.microPublication biology · 2026Article
- Modulating the pH dependent photophysical properties of green fluorescent protein.RSC advances · 2024Article
- Selective and Site-Specific Incorporation of Nonstandard Amino Acids Within Proteins for Therapeutic Applications.Methods in molecular biology (Clifton, N.J.) · 2024Article
- High-Throughput Aminoacyl-tRNA Synthetase Engineering for Genetic Code Expansion in Yeast.ACS synthetic biology · 2022Article
- Structural and spectrophotometric investigation of two unnatural amino-acid altered chromophores in the superfolder green fluorescent protein.Acta crystallographica. Section D, Structural biology · 2021Article
- Reprogramming the genetic code.Nature reviews. Genetics · 2021Review
- Dissecting Optical Response and Molecular Structure of Fluorescent Proteins With Non-canonical Chromophores.Frontiers in molecular biosciences · 2020Article
- Electron-deficient p-benzoyl-l-phenylalanine derivatives increase covalent chemical capture yields for protein-protein interactions.Protein science : a publication of the Protein Society · 2019Article
- Plasticity and Constraints of tRNA Aminoacylation Define Directed Evolution of Aminoacyl-tRNA Synthetases.International journal of molecular sciences · 2019Review
- Playing with the Molecules of Life.ACS chemical biology · 2018Review
- Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids.Molecules (Basel, Switzerland) · 2017Article
- A simple mathematical approach to the analysis of polypharmacology and polyspecificity data.F1000Research · 2017Article
- Utilization of alkyne bioconjugations to modulate protein function.Bioorganic & medicinal chemistry letters · 2017Article
- Synthesis and incorporation of a caged tyrosine amino acid possessing a bioorthogonal handle.Tetrahedron letters · 2016Article
- Expanding the scope of alkyne-mediated bioconjugations utilizing unnatural amino acids.Chemical communications (Cambridge, England) · 2016Article
- Synthesis and Incorporation of Unnatural Amino Acids To Probe and Optimize Protein Bioconjugations.Bioconjugate chemistry · 2015Article
- Gleaning unexpected fruits from hard-won synthetases: probing principles of permissivity in non-canonical amino acid-tRNA synthetases.Chembiochem : a European journal of chemical biology · 2014Article
- Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness.Nature chemical biology · 2014Article
- The "gate keeper" role of Trp222 determines the enantiopreference of diketoreductase toward 2-chloro-1-phenylethanone.PloS one · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The site-specific incorporation of unnatural amino acids (UAAs) into proteins in bacteria is made possible by the evolution of aminoacyl-tRNA synthetases that selectively recognize and aminoacylate the amino acid of interest. Recently we have discovered that some of the previously evolved aaRSs display a degree of polyspecificity and are capable of recognizing multiple UAAs. Herein we report the polyspecificity of an aaRS evolved to encode a comarin containing amino acid. This polyspecificity was then exploited to introduce several UAAs into the fluorophore of GFP, altering its photophysical properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.