ArticleVirology journal2011
Strategies to facilitate the development of uncloned or cloned infectious full-length viral cDNAs: Apple chlorotic leaf spot virus as a case study.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Reverse genetics platform for tomato mottle mosaic virus: infectious clone construction and resistance screening in tomato.Molecular biology reports · 2026Article
- Construction and Characterization of a Full-Length Infectious cDNA Clone of a Strain of Watermelon Mosaic Virus Isolated from Melon.The plant pathology journal · 2024Article
- Generation of a Triple-Shuttling Vector and the Application in Plant Plus-Strand RNA Virus Infectious cDNA Clone Construction.International journal of molecular sciences · 2023Article
- Identification of Seven Additional Genome Segments of Grapevine-Associated Jivivirus 1.Viruses · 2022Article
- Procedures and potential pitfalls for constructing a bee-infecting RNA virus clone.Frontiers in insect science · 2022Review
- Construction of Infectious Clones of Begomoviruses: Strategies, Techniques and Applications.Biology · 2021Review
- Synthesis of Full-Length cDNA Infectious Clones ofViruses · 2020Article
- Review
- Unravelling the virome in birch: RNA-Seq reveals a complex of known and novel viruses.PloS one · 2020Article
- Home-made enzymatic premix and Illumina sequencing allow for one-step Gibson assembly and verification of virus infectious clones.Phytopathology research · 2020Article
- Harnessed viruses in the age of metagenomics and synthetic biology: an update on infectious clone assembly and biotechnologies of plant viruses.Plant biotechnology journal · 2019Review
- Development of Soybean Yellow Mottle Mosaic Virus-Based Expression Vector for Heterologous Protein Expression in French Bean.Molecular biotechnology · 2019Article
- Agroinoculation of Grapevine Pinot Gris Virus in tobacco and grapevine provides insights on viral pathogenesis.PloS one · 2019Article
- Generation of an Infectious Clone of a New Korean Isolate ofThe plant pathology journal · 2017Article
- Article
- Generation of stable infectious clones of plant viruses by using Rhizobium radiobacter for both cloning and inoculation.Virology · 2017Article
- Movement protein of Apple chlorotic leaf spot virus is genetically unstable and negatively regulated by Ribonuclease E in E. coli.Scientific reports · 2017Article
- Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning.Viruses · 2015Article
- Gibson assembly: an easy way to clone potyviral full-length infectious cDNA clones expressing an ectopic VPg.Virology journal · 2015Article
- Yeast recombination-based cloning as an efficient way of constructing vectors for Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundApproaches to simplify and streamline the construction of full-length infectious cDNA clones (FL-cDNAs) are needed. Among desirable improvements are the ability to use total nucleic acids (TNA) extracts from infected hosts (to bypass viral purification limitations) for the direct one-step amplification of large FL-cDNAs, the possibility to inoculate plants with uncloned FL-cDNAs and the simplified cloning of these large molecules.
resultsUsing the 7.55 kb genome of Apple chlorotic leaf spot trichovirus (ACLSV) approaches allowing the rapid generation from TNA extracts of FL-cDNAs under the control of the T7 promoter and the successful inoculation of plants using in vitro transcripts obtained from these uncloned amplification products have been developed. We also show that the yeast homologous recombination system permits efficient cloning of FL-cDNAs and the simultaneous one-step tailoring of a ternary Yeast-Escherichia coli-Agrobacterium tumefaciens shuttle vector allowing efficient inoculation of both herbaceous and woody host plants by agroinfiltration.
conclusionsThe fast and efficient strategies described here should have broad applications, in particular for the study of "difficult" plant viruses, such as those infecting woody hosts, and potentially for other, non plant-infecting viral agents.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.