ArticleVirology journal2011
Thermal stability of hepatitis E virus assessed by a molecular biological approach.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 93 citations in OpenAlex.
- Feeding of the probiotic bacterium Enterococcus faecium NCIMB 10415 differentially affects shedding of enteric viruses in pigs.Veterinary research · 2012Trial
- Review
- Effectiveness of Cold Smoking on Inactivating Murine Norovirus in Salami-Like Pork Sausages (Mettwurst), and Hepatitis E Virus and Murine Norovirus in Solution.Food and environmental virology · 2025Article
- Distinct chikungunya virus polymerase palm subdomains contribute to viral protein accumulation and virion production.PLoS pathogens · 2024Article
- Seroprevalence survey of Hepatitis E Virus in Domestic Pigs in Guangdong, China.Animals : an open access journal from MDPI · 2024Article
- Changes in Hepatitis E Virus Contamination during the Production of Liver Sausage from Naturally Contaminated Pig Liver and the Potential of Individual Production Parameters to Reduce Hepatitis E Virus Contamination in the Processing Chain.Pathogens (Basel, Switzerland) · 2024Article
- A Multifaceted Approach for Evaluating Hepatitis E Virus Infectivity In Vitro: Cell Culture and Innovative Molecular Methods for Integrity Assessment.Veterinary sciences · 2023Article
- Development of an Ex Vivo Assay for Identification of Infectious Hepatitis E Virus in Different Kinds of Food Samples.Pathogens (Basel, Switzerland) · 2023Article
- Thermal Inactivation of Hepatitis E Virus in Pork Products Estimated with a Semiquantitative Infectivity Assay.Microorganisms · 2023Article
- Quantitative Risk Evaluation of Adventitious Agents in Heparin.TH open : companion journal to thrombosis and haemostasis · 2023Review
- Discrimination and surveillance of infectious severe acute respiratory syndrome Coronavirus 2 in wastewater using cell culture and RT-qPCR.The Science of the total environment · 2022Article
- Risk assessment of hepatitis E transmission through tissue allografts.World journal of gastrointestinal pathophysiology · 2022Review
- Real-Time PCR-Based Methods for Detection of Hepatitis E Virus in Pork Products: A Critical Review.Microorganisms · 2022Review
- A triton X-100 assisted PMAxx-qPCR assay for rapid assessment of infectious African swine fever virus.Frontiers in microbiology · 2022Article
- Cell Culture Isolation and Whole Genome Characterization of Hepatitis E Virus Strains from Wild Boars in Germany.Microorganisms · 2021Article
- Hepatitis E: Genotypes, strategies to prevent and manage, and the existing knowledge gaps.JGH open : an open access journal of gastroenterology and hepatology · 2021Review
- The Foodborne Transmission of Hepatitis E Virus to Humans.Food and environmental virology · 2021Review
- Article
- Technical and Anatomical Considerations for Reproducible Inactivation of Large Animal Carcasses by Steam Sterilization.Applied biosafety : journal of the American Biological Safety Association · 2021Article
- Optimization and Implementation of the Virus Extraction Method for Hepatitis E Virus Detection from Raw Pork Liver.Food and environmental virology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHepatitis E virus (HEV) is a pathogen of emerging concern in industrialized countries. The consumption of wild boar meat has been identified as one risk factor for autochthonous HEV infections. Only limited information is available about thermal stability of HEV, mainly due to the lack of rapid and efficient cell culture systems for measurement of HEV infectivity.
methodsA molecular biological method was implemented in order to distinguish disassembled from intact viral particles using RNase treatment followed by quantitative real-time RT-PCR. The method was applied to a wild boar liver suspension containing HEV genotype 3.
resultsTime-course analyses indicated that the decline of protected RNA could be described by a biphasic model with an initial decrease followed by a stationary phase. The stationary phase was reached after 1 hour at 4°C, 3 days at 22°C and 7 days at 37°C with log reductions of 0.34, 0.45 and 1.24, respectively. Protected RNA was detectable until the end of the experiments at day 50 or 70. Heat exposure for 1 minute resulted in a log reduction of 0.48 at 70°C and increased with higher temperatures to 3.67 at 95°C. Although HEV infectivity titration by inoculation of the liver suspension onto three cell lines did not succeed, the results of the RNase-based method are in accordance with published cell culture-based data.
conclusionsMeasurement of intact viral particles using the RNase-based method may provide data on the stability of RNA viruses when cell culture-based infectivity titrations are not efficient or not available. The method enables processing of large sample numbers and may be suitable to estimate stability of HEV in different types of food.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.