ArticleAnalytical chemistry2011
Microfluidic electrochemical array for detection of reactive metabolites formed by cytochrome P450 enzymes.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Microfluidics-Based Nanobiosensors for Healthcare Monitoring.Molecular biotechnology · 2024Review
- Evaluation of DNA adduct damage using G-quadruplex-based DNAzyme.Bioactive materials · 2023Article
- Electrochemiluminescence Arrays for Studies of Metabolite-related Toxicity.Electroanalysis · 2016Article
- Application of nanodisc technology for direct electrochemical investigation of plant cytochrome P450s and their NADPH P450 oxidoreductase.Scientific reports · 2016Article
- State-of-the-Art Metabolic Toxicity Screening and Pathway Evaluation.Analytical chemistry · 2016Article
- Ruthenium polypyridine complexes combined with oligonucleotides for bioanalysis: a review.Molecules (Basel, Switzerland) · 2014Review
- High-throughput metabolic genotoxicity screening with a fluidic microwell chip and electrochemiluminescence.Lab on a chip · 2013Article
- Paper-based electrochemiluminescent screening for genotoxic activity in the environment.Environmental science & technology · 2013Article
- Screening reactive metabolites bioactivated by multiple enzyme pathways using a multiplexed microfluidic system.The Analyst · 2013Article
- Electrochemical Activation of the Natural Catalytic Cycle of Cytochrome P450s in Human Liver Microsomes.Electroanalysis · 2012Article
- Elucidating Organ-Specific Metabolic Toxicity Chemistry from Electrochemiluminescent Enzyme/DNA Arrays and Bioreactor Bead-LC-MS/MS.Chemical scienceArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
A novel, simple, rapid microfluidic array using bioelectronically driven cytochrome P450 enzyme catalysis for reactive metabolite screening is reported for the first time. The device incorporates an eight-electrode screen-printed carbon array coated with thin films of DNA, [Ru(bpy)(2)(PVP)(10)](ClO(4)) {RuPVP}, and rat liver microsomes (RLM) as enzyme sources. Catalysis features electron donation to cyt P450 reductase in the RLMs and subsequent cyt P450 reduction while flowing an oxygenated substrate solution past sensor electrodes. Metabolites react with DNA in the film if they are able, and damaged DNA is detected by catalytic square wave voltammetry (SWV) utilizing the RuPVP polymer. The microfluidic device was tested for a set of common pollutants known to form DNA-reactive metabolites. Logarithmic turnover rates based on SWV responses gave excellent correlation with the rodent liver TD(50) toxicity metric, supporting the utility of the device for toxicity screening. The microfluidic array gave much better S/N and reproducibility than single-electrode sensors based on similar principles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.