ArticleMolecular microbiology2011
An amino-terminal signal peptide of Vfr protein negatively influences RopB-dependent SpeB expression and attenuates virulence in Streptococcus pyogenes.
Article in Molecular microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 34 citations in OpenAlex.
- A protease-sensing circuit links neutrophil inflammation to virulence regulation inbioRxiv : the preprint server for biology · 2026Article
- Rgg/SHP transcriptional regulators, RaS-RiPPs, and their impacts in streptococci.F1000Research · 2026Review
- The leaderless communication peptide (LCP) class of quorum-sensing peptides is broadly distributed among Firmicutes.Nature communications · 2023Article
- Hyperphosphorylation of the Group A Streptococcal Control of Virulence Regulator Increases Promoter Occupancy Specifically at Virulence Factor-Encoding Genes.Journal of bacteriology · 2023Article
- RopB represses the transcription ofLife science alliance · 2023Article
- Signaling Peptide SpoV Is Essential forMicroorganisms · 2021Article
- TheJournal of bacteriology · 2021Article
- In silico characterisation of stand-alone response regulators of Streptococcus pyogenes.PloS one · 2020Article
- Environmental pH and peptide signaling control virulence of Streptococcus pyogenes via a quorum-sensing pathway.Nature communications · 2019Article
- Signaling by a Conserved Quorum Sensing Pathway Contributes to GrowthInfection and immunity · 2018Article
- Endopeptidase PepO Regulates the SpeB Cysteine Protease and Is Essential for the Virulence of Invasive M1T1 Streptococcus pyogenes.Journal of bacteriology · 2018Article
- A novel chemical inducer ofThe Journal of biological chemistry · 2018Article
- Leaderless secreted peptide signaling molecule alters global gene expression and increases virulence of a human bacterial pathogen.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Structural Mechanisms of Peptide Recognition and Allosteric Modulation of Gene Regulation by the RRNPP Family of Quorum-Sensing Regulators.Journal of molecular biology · 2016Review
- Structural and functional analysis of RopB: a major virulence regulator in Streptococcus pyogenes.Molecular microbiology · 2016Article
- Induction of a quorum sensing pathway by environmental signals enhances group A streptococcal resistance to lysozyme.Molecular microbiology · 2015Article
- Phosphorylation events in the multiple gene regulator of group A Streptococcus significantly influence global gene expression and virulence.Infection and immunity · 2015Article
- An ABC transporter is required for secretion of peptide sex pheromones in Enterococcus faecalis.mBio · 2014Article
- Peptide pheromone signaling in Streptococcus and Enterococcus.FEMS microbiology reviews · 2014Review
- Quorum sensing in group A Streptococcus.Frontiers in cellular and infection microbiology · 2014Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Streptococcal pyrogenic exotoxin B (SpeB) is an extracellular cysteine protease that is a critical virulence factor made by the major human pathogen group A Streptococcus (GAS). speB expression is dependent on the regulator of proteinase B (RopB) and is upregulated with increasing cell density and during infection. Because computer modelling suggested significant structural similarity between RopB and peptide-sensing regulatory proteins made by other Gram-positive bacteria, we hypothesized that speB expression is influenced by RopB-peptide interactions. Inactivation of the gene (vfr) encoding the virulence factor related (Vfr) protein resulted in increased speB transcript level during the exponential growth phase, whereas provision of only the amino-terminal region of Vfr comprising the secretion signal sequence in trans restored a wild-type speB expression profile. Addition of the culture supernatant from a Vfr signal peptide-expressing GAS strain restored wild-type speB transcript level to a vfr-inactivated isogenic mutant strain. A distinct peptide in the Vfr secretion signal sequence specifically bound to recombinant RopB. Finally, overexpression of the Vfr secretion signal sequence significantly decreased speB transcript level and attenuated GAS virulence in two mouse models of invasive infection. Taken together, these data delineate a previously unknown small peptide-mediated regulatory system that controls GAS virulence factor production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.