ArticlePLoS neglected tropical diseases2011
Alternative oxidase mediates pathogen resistance in Paracoccidioides brasiliensis infection.
Article in PLoS neglected tropical diseases, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
- Review
- Inhibition of mitosomal alternative oxidase causes lifecycle arrest of early-stage Trachipleistophora hominis meronts during intracellular infection of mammalian cells.PLoS pathogens · 2022Article
- Updates inJournal of fungi (Basel, Switzerland) · 2021Review
- One Century of Study: What We Learned aboutJournal of fungi (Basel, Switzerland) · 2021Review
- Alternative Oxidase: A Potential Target for Controlling Aflatoxin Contamination and Propagation ofFrontiers in microbiology · 2020Review
- The Trojan Horse Model inFrontiers in cellular and infection microbiology · 2020Review
- Down-regulation of TUFM impairs host cell interaction and virulence by Paracoccidioides brasiliensis.Scientific reports · 2019Article
- Involvement of a G Protein Regulatory Circuit in Alternative Oxidase Production inG3 (Bethesda, Md.) · 2019Article
- Insulin ModulatesJournal of diabetes research · 2018Article
- Depicting the metabolism of Paracoccidioides brasiliensis during infection by transcriptional and proteomic approaches.Virulence · 2017Article
- Paracoccidioides spp. catalases and their role in antioxidant defense against host defense responses.Fungal genetics and biology : FG & B · 2017Article
- Article
- Review
- A Functional Approach towards Understanding the Role of the Mitochondrial Respiratory Chain in an Endomycorrhizal Symbiosis.Frontiers in plant science · 2017Article
- Identification and Analysis of the Role of Superoxide Dismutases Isoforms in the Pathogenesis of Paracoccidioides spp.PLoS neglected tropical diseases · 2016Article
- Decreased expression of 14-3-3 in Paracoccidioides brasiliensis confirms its involvement in fungal pathogenesis.Virulence · 2016Article
- Characterization of the Paracoccidioides Hypoxia Response Reveals New Insights into Pathogenesis Mechanisms of This Important Human Pathogenic Fungus.PLoS neglected tropical diseases · 2015Article
- Involvement of an alternative oxidase in the regulation of hyphal growth and microsclerotial formation in Nomuraea rileyi CQNr01.World journal of microbiology & biotechnology · 2015Article
- Genome update of the dimorphic human pathogenic fungi causing paracoccidioidomycosis.PLoS neglected tropical diseases · 2014Article
- Paracoccidioides species complex: ecology, phylogeny, sexual reproduction, and virulence.PLoS pathogens · 2014Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundParacoccidioides brasiliensis is a human thermal dimorphic pathogenic fungus. Survival of P. brasiliensis inside the host depends on the adaptation of this fungal pathogen to different conditions, namely oxidative stress imposed by immune cells. AIMS AND METHODOLOGY: In this study, we evaluated the role of alternative oxidase (AOX), an enzyme involved in the intracellular redox balancing, during host-P. brasiliensis interaction. We generated a mitotically stable P. brasiliensis AOX (PbAOX) antisense RNA (aRNA) strain with a 70% reduction in gene expression. We evaluated the relevance of PbAOX during interaction of conidia and yeast cells with IFN-γ activated alveolar macrophages and in a mouse model of infection. Additionally, we determined the fungal cell's viability and PbAOX in the presence of H₂O₂.
resultsInteraction with IFN-γ activated alveolar macrophages induced higher levels of PbAOX gene expression in PbWt conidia than PbWt yeast cells. PbAOX-aRNA conidia and yeast cells had decreased viability after interaction with macrophages. Moreover, in a mouse model of infection, we showed that absence of wild-type levels of PbAOX in P. brasiliensis results in a reduced fungal burden in lungs at weeks 8 and 24 post-challenge and an increased survival rate. In the presence of H₂O₂, we observed that PbWt yeast cells increased PbAOX expression and presented a higher viability in comparison with PbAOX-aRNA yeast cells.
conclusionsThese data further support the hypothesis that PbAOX is important in the fungal defense against oxidative stress imposed by immune cells and is relevant in the virulence of P. brasiliensis.
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