ArticlePloS one2011
Molecular analysis of virulent determinants of enterovirus 71.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
55 citing papers in PubMed, 93 citations in OpenAlex.
- Complete genome sequencing and molecular characterization of indigenous enterovirus A71 genogroups D and G of India.Virology journal · 2026Article
- Update on enteroviral protease 2A: Structure, function, and host factor interaction.Biosafety and health · 2023Review
- The effects of SCARB2 and SELPLG gene polymorphisms on EV71 infection in hand, foot and mouth disease.Biomolecules & biomedicine · 2023Article
- Clinical and molecular epidemiologic features of enterovirus D68 infection in children with acute lower respiratory tract infection in China.Archives of virology · 2023Review
- Antibody signatures in hospitalized hand, foot and mouth disease patients with acute enterovirus A71 infection.PLoS pathogens · 2023Article
- Variant enterovirus A71 found in immune-suppressed patient binds to heparan sulfate and exhibits neurotropism in B-cell-depleted mice.Cell reports · 2023Article
- Amino acid variation at VP1-145 of enterovirus A71 determines the viral infectivity and receptor usage in a primary human intestinal model.Frontiers in microbiology · 2023Article
- Article
- Global emergence of Enterovirus 71: a systematic review.Beni-Suef University journal of basic and applied sciences · 2022Review
- Heat Shock Protein A6 Is Especially Involved in Enterovirus 71 Infection.Frontiers in microbiology · 2022Article
- Adaptation and Virulence of Enterovirus-A71.Viruses · 2021Review
- A virulent and pathogenic infectious clone of Senecavirus A.The Journal of general virology · 2021Article
- Molecular epidemiology of enteroviruses associated with severe hand, foot and mouth disease in Shenzhen, China, 2014-2018.Archives of virology · 2020Article
- Characterization of Plaque Variants and the Involvement of Quasi-Species in a Population of EV-A71.Viruses · 2020Article
- Heparan sulfate attachment receptor is a major selection factor for attenuated enterovirus 71 mutants during cell culture adaptation.PLoS pathogens · 2020Article
- Novel Naturally Occurring Mutations of Enterovirus 71 Associated With Disease Severity.Frontiers in microbiology · 2020Article
- Enterovirus and Encephalitis.Frontiers in microbiology · 2020Review
- Enterovirus A71: virulence, antigenicity, and genetic evolution over the years.Journal of biomedical science · 2019Review
- A decade of sustained selection pressure on two surface sites of the VP1 protein of Enterovirus A71 suggests that immune evasion may be an indirect driver for virulence.Scientific reports · 2019Article
- A Novel Live Attenuated Vaccine Candidate Protects Against HeterologousFrontiers in immunology · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterovirus 71 (EV71) is the most important causative agent of hand, foot and mouth disease (HFMD) in children. In most cases, it is a self-limiting illness. However some EV71 infectious cases can develop severe clinical outcomes, such as encephalitis, meningitis, poliomyelitis like paralysis, and even death. To identify the determinants of virulence, the deduced amino acid sequence of polyprotein and nucleotide sequence of 5'-NTR and 3'-NTR in 25 SC-EV71 strains (strains from severe cases) and 31 MC-EV71 strains (strains from mild cases) were analyzed. Results showed four amino acids on two positions (Gly(P710)/Gln(P710)/Arg(P710) and Glu(P729)) on the DE and EF loop of VP1, one (Lys(P930)) on the surface of protease 2A and four nucleotides on three positions (G(P272), U(P488) and A(P700)/U(P700)) in the 5'-NTR region are associated with EV71 virulent phenotype. Predicted secondary structure of RNA using the consensus sequence of 5'-NTR by RNAStructure showed the mutation of nucleotide at position 488 in strain BJ08-Z004-3 (position 491 in prototype strain BrCr) can result in the discrepancy of an additional pair of nucleotides and thus change the stability of the second structure of IRES. Fragment base content analysis showed that in the region 696 to 714 bp at the 5'-NTR, where the A(P700)/U(P700) was located, the nucleotide constitution ratios differed significantly between SC-EV71 and MC-EV71 strains. In conclusion, comparative genomic analysis showed that virulence of EV71 strains are mainly determined by the amino acids on two positions of VP1, one position of protease 2A and the nucleotides on three positions in 5'-NTR.
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