ArticlePloS one2011
Genomic analyses, gene expression and antigenic profile of the trans-sialidase superfamily of Trypanosoma cruzi reveal an undetected level of complexity.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed, 127 citations in OpenAlex.
- Vaccines against chronicExpert review of vaccines · 2026Review
- Beyond the catalysis of Trypanosoma cruzi trans-sialidases: structure, function, post-translational modifications, intrinsically disordered regions and use of CRISPR/Cas9.Archives of microbiology · 2026Review
- Single-cell RNA-seq reveals trans-sialidase-like superfamily gene expression heterogeneity ineLife · 2026Article
- Epitope mapping of vaccine antigens Tc24 and TSA1 with antibodies from Trypanosoma cruzi-infected patients.Genes and immunity · 2026Article
- Transcriptomic dynamics reveals sequential acquisition of complement resistance during prolonged starvation of Trypanosoma cruzi epimastigoteMemorias do Instituto Oswaldo Cruz · 2026Article
- Distinct nanoscale membrane organizations of mucins andbioRxiv : the preprint server for biology · 2026Article
- The complete genome of Trypanosoma cruzi reveals 32 chromosomes and three genomic compartments.BMC genomics · 2026Article
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- Nuclear Genome Assembly and Annotation of Kinetoplastids.Methods in molecular biology (Clifton, N.J.) · 2026Article
- An Algorithm for Annotation of Trypanosoma cruzi MASP Sequences.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A divide-and-conquer approach to uncover the genomic structure of the highly virulent RA strain of Trypanosoma cruzi.Scientific reports · 2025Article
- Variation in surface protein expression leads to heterogeneous Trypanosoma cruzi populations during host cell infection.Nature communications · 2025Article
- Cardiac alterations induced by Trypanosoma cruzi extracellular vesicles and immune complexes.PLoS neglected tropical diseases · 2025Article
- Mass spectrometry-based discovery and diagnostic validation of T. cruzi antigens in the urine of congenitally infected Chagas Disease patients.PLoS neglected tropical diseases · 2025Article
- An algorithm for annotation and classification of T. cruzi MASP sequences: towards a better understanding of the parasite genetic variability.BMC genomics · 2025Article
- Review
- RNA and protein immunization withFrontiers in cellular and infection microbiology · 2025Article
- Nasal vaccination againstFrontiers in immunology · 2025Article
- Towards a map of the immune system manipulation network byFrontiers in cellular and infection microbiology · 2025Review
- Circulating extracellular vesicles in sera of chronic patients as a method for determining active parasitism in Chagas disease.PLoS neglected tropical diseases · 2024Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The protozoan parasite Trypanosoma cruzi is the etiologic agent of Chagas disease, a highly debilitating human pathology that affects millions of people in the Americas. The sequencing of this parasite's genome reveals that trans-sialidase/trans-sialidase-like (TcS), a polymorphic protein family known to be involved in several aspects of T. cruzi biology, is the largest T. cruzi gene family, encoding more than 1,400 genes. Despite the fact that four TcS groups are well characterized and only one of the groups contains active trans-sialidases, all members of the family are annotated in the T. cruzi genome database as trans-sialidase. After performing sequence clustering analysis with all TcS complete genes, we identified four additional groups, demonstrating that the TcS family is even more heterogeneous than previously thought. Interestingly, members of distinct TcS groups show distinctive patterns of chromosome localization. Members of the TcSgroupII, which harbor proteins involved in host cell attachment/invasion, are preferentially located in subtelomeric regions, whereas members of the largest and new TcSgroupV have internal chromosomal locations. Real-time RT-PCR confirms the expression of genes derived from new groups and shows that the pattern of expression is not similar within and between groups. We also performed B-cell epitope prediction on the family and constructed a TcS specific peptide array, which was screened with sera from T. cruzi-infected mice. We demonstrated that all seven groups represented in the array are antigenic. A highly reactive peptide occurs in sixty TcS proteins including members of two new groups and may contribute to the known cross-reactivity of T. cruzi epitopes during infection. Taken together, our results contribute to a better understanding of the real complexity of the TcS family and open new avenues for investigating novel roles of this family during T. cruzi infection.
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