ArticleInvestigative ophthalmology & visual science2011
Increase of intracellular Ca2+ by purinergic receptors in cultured rat lacrimal gland myoepithelial cells.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 37 citations in OpenAlex.
- Purinergic inhibitory regulation of esophageal smooth muscle is mediated by P2Y receptors and ATP-dependent potassium channels in rats.The journal of physiological sciences : JPS · 2024Article
- Interleukin-1β activates matrix metalloproteinase-2 to alter lacrimal gland myoepithelial cell structure and function.Frontiers in ophthalmology · 2024Article
- Genomic DNA activates the AIM2 inflammasome and STING pathways to induce inflammation in lacrimal gland myoepithelial cells.The ocular surface · 2023Article
- Role of the adenylate cyclase/cyclic AMP pathway in oxytocin-induced lacrimal gland myoepithelial cells contraction.Experimental eye research · 2023Article
- Slow hydrogel matrix degradation enhances salivary gland mimetic phenotype.Acta biomaterialia · 2023Article
- Effects of proinflammatory cytokines on lacrimal gland myoepithelial cells contraction.Frontiers in ophthalmology · 2022Article
- Role of the Phospholipase C Pathway and Calcium Mobilization in Oxytocin-Induced Contraction of Lacrimal Gland Myoepithelial Cells.Investigative ophthalmology & visual science · 2021Article
- Receptor-specific CaiScience · 2021Article
- Novel Insights Into Muscarinic and Purinergic Responses in Primary Cultures of Rat Lacrimal Gland Myoepithelial Cells.Investigative ophthalmology & visual science · 2021Article
- Article
- Lacrimal Gland Myoepithelial Cells Are Altered in a Mouse Model of Dry Eye Disease.The American journal of pathology · 2020Article
- Dissecting the cellular specificity of smoking effects and reconstructing lineages in the human airway epithelium.Nature communications · 2020Article
- A Primary Cell and Organoid Platform for Evaluating Pharmacological Responses in Mammary Epithelial Cells.ACS pharmacology & translational science · 2020Article
- P2 Receptors as Therapeutic Targets in the Salivary Gland: From Physiology to Dysfunction.Frontiers in pharmacology · 2020Review
- Intravital Two-photon Imaging of CaScientific reports · 2018Article
- Myoepithelial cell-driven acini contraction in response to oxytocin receptor stimulation is impaired in lacrimal glands of Sjögren's syndrome animal models.Scientific reports · 2018Article
- Alteration in nerves and neurotransmitter stimulation of lacrimal gland secretion in the TSP-1Mucosal immunology · 2018Article
- Bottom-up assembly of salivary gland microtissues for assessing myoepithelial cell function.Biomaterials · 2017Article
- Alteration in cellular turnover and progenitor cell population in lacrimal glands from thrombospondin 1Experimental eye research · 2016Article
- Ocular Purine Receptors as Drug Targets in the Eye.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2016Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
purposeTo isolate and characterize cultured myoepithelial cells (MECs) from rat lacrimal gland and determine which purinergic receptor subtypes are present and functional in MECs.
methodsRat lacrimal glands were subjected to collagenase digestion, and MECs were grown. RT-PCR was performed for the purinergic receptors P2X(7), P2Y(1), P2Y(11), and P2Y(13) on RNA isolated from the MECs. Immunofluorescence experiments were performed with antibodies against MEC markers and P2X(7), P2Y(1), P2Y(11), and P2Y(13) purinergic receptors. Proteins from MECs were separated using Western blot analysis techniques. In addition, cells were incubated with Fura 2 tetra acetoxymethyl ester, and intracellular [Ca(2+)] ([Ca(2+)](i)) was determined in response to P2 purinergic agonists.
resultsMECs expressed the MEC proteins α-smooth muscle actin, vimentin, α-actinin, and adenylyl cyclase II. RT-PCR, Western blot, and immunofluorescence techniques demonstrated the presence of the purinergic receptors P2X(7), P2Y(1), P2Y(11), and P2Y(13). The purinergic agonists ATP, benzoylbenzoyl ATP (BzATP), α,β methylene ATP, UTP, 2-methylthioATP (MeSATP), and ATPγS increased [Ca(2+)](i). As BzATP binds to the P2X(7) receptor, specific characteristics of this receptor were investigated. Neither inhibitors of P2X(7) receptors nor removal of extracellular Mg(2+) or Ca(2+) had an effect on the BzATP-stimulated increase in [Ca(2+)](i). Repeated applications of BzATP desensitized this response. Inhibitors for P2Y(1), P2Y(11), and P2Y(13) each decreased the BzATP-stimulated increase in [Ca(2+)](i) with the P2Y(1) inhibitor most effective.
conclusionsMECs can be isolated from rat lacrimal glands, and they express P2X(7), P2Y(1), P2Y(11), and P2Y(13) purinergic receptors. Surprisingly, BzATP binds the P2Y(1) receptor, which is primarily responsible for the BzATP-stimulated increase in [Ca(2+)](i).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.