ArticlePlant physiology2012
Mapping, complementation, and targets of the cysteine protease actinidin in kiwifruit.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 34 citations in OpenAlex.
- Influence of drought stress on phosphorus dynamics and maize growth in tropical ecosystems.BMC plant biology · 2025Article
- Cysteine Peptidases ofCurrent protein & peptide science · 2025Review
- Acknowledging the use of botanicals to treat diabetic foot ulcer during the 21World journal of clinical cases · 2023Article
- Habitual Green Kiwifruit Consumption Is Associated with a Reduction in Upper Gastrointestinal Symptoms: A Systematic Scoping Review.Advances in nutrition (Bethesda, Md.) · 2022Article
- Illustration of the variation in the content of flavanone rutinosides in various citrus germplasms from genetic and enzymatic perspectives.Horticulture research · 2022Article
- A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing.Molecular biology reports · 2021Article
- Protuberances are organized distinct regions of long-term callus: histological and transcriptomic analyses in kiwifruit.Plant cell reports · 2021Article
- Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall.Frontiers in plant science · 2020Article
- Effects of Topical Kiwifruit on Healing of Chronic Bedsore.The Indian journal of surgery · 2015Article
- Effects of topical Kiwifruit on healing of neuropathic diabetic foot ulcer.Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2014Article
- Functional properties of a cysteine proteinase from pineapple fruit with improved resistance to fungal pathogens in Arabidopsis thaliana.Molecules (Basel, Switzerland) · 2014Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cysteine proteases (CPs) accumulate to high concentration in many fruit, where they are believed to play a role in fungal and insect defense. The fruit of Actinidia species (kiwifruit) exhibit a range of CP activities (e.g. the Actinidia chinensis variety YellowA shows less than 2% of the activity of Actinidia deliciosa variety Hayward). A major quantitative trait locus for CP activity was mapped to linkage group 16 in a segregating population of A. chinensis. This quantitative trait locus colocated with the gene encoding actinidin, the major acidic CP in ripe Hayward fruit encoded by the ACT1A-1 allele. Sequence analysis indicated that the ACT1A locus in the segregating A. chinensis population contained one functional allele (A-2) and three nonfunctional alleles (a-3, a-4, and a-5) each containing a unique frameshift mutation. YellowA kiwifruit contained two further alleles: a-6, which was nonfunctional because of a large insertion, and a-7, which produced an inactive enzyme. Site-directed mutagenesis of the act1a-7 protein revealed a residue that restored CP activity. Expression of the functional ACT1A-1 cDNA in transgenic plants complemented the natural YellowA mutations and partially restored CP activity in fruit. Two consequences of the increase in CP activity were enhanced degradation of gelatin-based jellies in vitro and an increase in the processing of a class IV chitinase in planta. These results provide new insight into key residues required for CP activity and the in vivo protein targets of actinidin.
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