ArticleTransgenic research2012
The creation of transgenic pigs expressing human proteins using BAC-derived, full-length genes and intracytoplasmic sperm injection-mediated gene transfer.
Article in Transgenic research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Application of the transgenic pig model in biomedical research: A review.Frontiers in cell and developmental biology · 2022Review
- Supplementation of fetal bovine serum increased the quality ofJournal of advanced veterinary and animal research · 2021Article
- A New Toolbox in Experimental Embryology-Alternative Model Organisms for Studying Preimplantation Development.Journal of developmental biology · 2021Review
- Chaetocin Improves Pig Cloning Efficiency by Enhancing Epigenetic Reprogramming and Autophagic Activity.International journal of molecular sciences · 2020Article
- Enhanced Production of Herpes Simplex Virus 1 Amplicon Vectors by Gene Modification and Optimization of Packaging Cell Growth Medium.Molecular therapy. Methods & clinical development · 2020Article
- Embryo aggregation regulatesPeerJ · 2019Article
- Direct introduction of gene constructs into the pronucleus-like structure of cloned embryos: a new strategy for the generation of genetically modified pigs.Transgenic research · 2017Article
- Dual effect of fetal bovine serum on early development depends on stage-specific reactive oxygen species demands in pigs.PloS one · 2017Article
- Production of Transgenic Pigs with an Introduced Missense Mutation of the Bone Morphogenetic Protein Receptor Type IB Gene Related to Prolificacy.Asian-Australasian journal of animal sciences · 2016Article
- Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new.Biology direct · 2016Review
- Comparison of chemical, electrical, and combined activation methods for in vitro matured porcine oocytes.In vitro cellular & developmental biology. Animal · 2015Article
- Generation of transgenic pigs by cytoplasmic injection of piggyBac transposase-based pmGENIE-3 plasmids.Biology of reproduction · 2014Article
- Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons.Nature protocols · 2014Article
- Transgenic pigs with pancreas-specific expression of green fluorescent protein.The Journal of reproduction and development · 2014Article
- Production of pigs expressing a transgene under the control of a tetracycline-inducible system.PloS one · 2014Article
- Article
- Precision editing of large animal genomes.Advances in genetics · 2012Review
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In most transgenic (Tg) animals created to date, a transgene consisting of the minimum promoter region linked to a cDNA has been used. However, transgenes on small plasmids are susceptible to the position effect, increasing the difficulty of controlling transgene expression. In this study, we attempted to create Tg pigs by intracytoplasmic sperm injection-mediated gene transfer (ICSI-MGT) using two large genomic transgenes derived from a bacterial artificial chromosome (BAC) containing the full genomic region encoding two human proteins, type I collagen and albumin. The production efficiencies (Tg piglets/live offspring) of type I collagen and albumin Tg pigs were 11.8% (6/51) and 18.2% (2/11), respectively. In all of the Tg pigs examined by real-time PCR analysis, tissue-specific expression of the transgene was confirmed (type I collagen: skin, tendon, vessels, genitalia; albumin: liver). The production of human proteins derived from BAC transgenes was also confirmed. Fluorescence in situ hybridization analysis indicated that the BAC transgenes transferred into porcine oocytes by ICSI-MGT were integrated into single or multiple sites on the host chromosomes. These data demonstrate that Tg pigs expressing human proteins in a tissue-specific manner can be created using a BAC transgenic construct and the ICSI-MGT method.
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Registered trials
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