ArticlePlant molecular biology2011
Analysis of raphidophyte assimilatory nitrate reductase reveals unique domain architecture incorporating a 2/2 hemoglobin.
Article in Plant molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- Effects of nitrate and ammonium on assimilation of nitric oxide by Heterosigma akashiwo.Scientific reports · 2023Article
- Nitrate reductase enzymes in algaFrontiers in microbiology · 2023Article
- Distinctive structural properties of THB11, a pentacoordinate Chlamydomonas reinhardtii truncated hemoglobin with N- and C-terminal extensions.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2020Article
- Strategies among phytoplankton in response to alleviation of nutrient stress in a subtropical gyre.The ISME journal · 2019Article
- Evolution and regulation of nitrogen flux through compartmentalized metabolic networks in a marine diatom.Nature communications · 2019Article
- Expression of novel nitrate reductase genes in the harmful alga, Chattonella subsalsa.Scientific reports · 2018Article
- Covalent attachment of the heme to Synechococcus hemoglobin alters its reactivity toward nitric oxide.Journal of inorganic biochemistry · 2017Article
- Structure of Chlamydomonas reinhardtii THB1, a group 1 truncated hemoglobin with a rare histidine-lysine heme ligation.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Understanding nitrate assimilation and its regulation in microalgae.Frontiers in plant science · 2015Review
- THB1 regulates nitrate reductase activity and THB1 and THB2 transcription differentially respond to NO and the nitrate/ammonium balance in Chlamydomonas.Plant signaling & behavior · 2015Article
- Evolution of mitochondria reconstructed from the energy metabolism of living bacteria.PloS one · 2014Article
- Hemoglobin: a nitric-oxide dioxygenase.Scientifica · 2012Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
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Abstract
Eukaryotic assimilatory nitrate reductase (NR) is a multi-domain protein that catalyzes the rate-limiting step in nitrate assimilation. This protein is highly conserved and has been extensively characterized in plants and algae. Here, we report hybrid NRs (NR2-2/2HbN) identified in two microalgal species, Heterosigma akashiwo and Chattonella subsalsa, with a 2/2 hemoglobin (2/2Hb) inserted into the hinge 2 region of a prototypical NR. 2/2Hbs are a class of single-domain heme proteins found in bacteria, ciliates, algae and plants. Sequence analysis indicates that the C-terminal FAD/NADH reductase domain of NR2-2/2HbN retains identity with eukaryotic NR, suggesting that the 2/2Hb domain was inserted interior to the existing NR domain architecture. Phylogenetic analysis supports the placement of the 2/2Hb domain of NR2-2/2HbN within group I (N-type) 2/2Hbs with high similarity to mycobacterial 2/2HbNs, known to convert nitric oxide to nitrate. Experimental data confirms that H. akashiwo is capable of metabolizing nitric oxide and shows that HaNR2-2/2HbN expression increases in response to nitric oxide addition. Here, we propose a mechanism for the dual function of NR2-2/2HbN in which nitrate reduction and nitric oxide dioxygenase reactions are cooperative, such that conversion of nitric oxide to nitrate is followed by reduction of nitrate for assimilation as cellular nitrogen.
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