ArticleNature methods2011
Automated protein-DNA interaction screening of Drosophila regulatory elements.
Article in Nature methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Modulating the levels and activity of the retinal determination network is sufficient to induce the specification of distinct tissue fates in Drosophila.Development (Cambridge, England) · 2025Article
- A genetic screen of transcription factors in the Drosophila melanogaster abdomen identifies novel pigmentation genes.G3 (Bethesda, Md.) · 2024Article
- Paired yeast one-hybrid assays to detect DNA-binding cooperativity and antagonism across transcription factors.Nature communications · 2023Article
- A modERN resource: identification of Drosophila transcription factor candidate target genes using RNAi.Genetics · 2023Article
- Enhanced Yeast One-Hybrid Assays to Study Protein-DNA Interactions.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A Tead1-Apelin axis directs paracrine communication from myogenic to endothelial cells in skeletal muscle.iScience · 2022Article
- A comprehensive temporal patterning gene network in Drosophila medulla neuroblasts revealed by single-cell RNA sequencing.Nature communications · 2022Article
- Large-scale data analysis for robotic yeast one-hybrid platforms and multi-disciplinary studies using GateMultiplex.BMC biology · 2021Article
- Article
- Unraveling the Genetic Basis for the Rapid Diversification of Male Genitalia between Drosophila Species.Molecular biology and evolution · 2021Article
- Evolutionary novelty in the apoptotic pathway of aphids.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Discovering human transcription factor physical interactions with genetic variants, novel DNA motifs, and repetitive elements using enhanced yeast one-hybrid assays.Genome research · 2019Article
- Microfluidic epigenomic mapping technologies for precision medicine.Lab on a chip · 2019Review
- A novel synthetic-genetic-array-based yeast one-hybrid system for high discovery rate and short processing time.Genome research · 2019Article
- A Comprehensive Drosophila melanogaster Transcription Factor Interactome.Cell reports · 2019Article
- Hierarchical Transcription Factor and Chromatin Binding Network for Wood Formation in Black Cottonwood (The Plant cell · 2019Article
- High-Throughput Yeast One-Hybrid Screens Using a Cell Surface gLUC Reporter.Current protocols in plant biology · 2019Article
- Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences.Journal of visualized experiments : JoVE · 2019Article
- Generation of a versatile BiFC ORFeome library for analyzing protein-protein interactions in liveeLife · 2018Article
- Eyeless/Pax6 initiates eye formation non-autonomously from the peripodial epithelium.Development (Cambridge, England) · 2018Article
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Authors and funding
9 authors.
Funding
Abstract
Drosophila melanogaster has one of the best characterized metazoan genomes in terms of functionally annotated regulatory elements. To explore how these elements contribute to gene regulation, we need convenient tools to identify the proteins that bind to them. Here we describe the development and validation of a high-throughput yeast one-hybrid platform, which enables screening of DNA elements versus an array of full-length, sequence-verified clones containing over 85% of predicted Drosophila transcription factors. Using six well-characterized regulatory elements, we identified 33 transcription factor-DNA interactions of which 27 were previously unidentified. To simultaneously validate these interactions and locate the binding sites of involved transcription factors, we implemented a powerful microfluidics-based approach that enabled us to retrieve DNA-occupancy data for each transcription factor throughout the respective target DNA elements. Finally, we biologically validated several interactions and identified two new regulators of sine oculis gene expression and hence eye development.
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