ArticleJournal of molecular biology2011
The scope of phage display for membrane proteins.
Article in Journal of molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Rational Design and Delivery of Engineered Peptides: Toward a Unified Framework for Structure, Screening, and Application.Biology · 2026Review
- Article
- Rapid On-Cell Selection of High-Performance Human Antibodies.ACS central science · 2022Article
- Directed evolution and biophysical characterization of a full-length, soluble, human caveolin-1 variant.Biochimica et biophysica acta. Proteins and proteomics · 2018Article
- Dissecting binding of a β-barrel membrane protein by phage display.Molecular bioSystems · 2017Article
- Antibody Engineering for Pursuing a Healthier Future.Frontiers in microbiology · 2017Review
- Chemically Modifying Viruses for Diverse Applications.ACS chemical biology · 2016Review
- Glutamate provides a key structural contact between reticulon-4 (Nogo-66) and phosphocholine.Biochimica et biophysica acta · 2014Article
- Construction of helper plasmid-mediated dual-display phage for autoantibody screening in serum.Applied microbiology and biotechnology · 2014Article
- High-throughput identification of putative receptors for cancer-binding peptides using biopanning and microarray analysis.Integrative biology : quantitative biosciences from nano to macro · 2013Article
- Selection of binding targets in parasites using phage-display and aptamer libraries in vivo and in vitro.Frontiers in immunology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Numerous examples of phage display applied to soluble proteins demonstrate the power of the technique for protein engineering, affinity reagent discovery and structure-function studies. Recent reports have expanded phage display to include membrane proteins (MPs). The scope and limitations of MP display remain undefined. Therefore, we report data from the phage display of representative types of membrane-associated proteins including plasma, nuclear, peripheral, single and multipass. The peripheral MP neuromodulin displays robustly with packaging by conventional M13-KO7 helper phage. The monotopic MP Nogo-66 can also display on the phage surface, if packaged by the modified M13-KO7(+) helper phage. The modified phage coat of KO7(+) can better mimic the zwitterionic character of the plasma membrane. Four examples of putatively α-helical, integral MPs failed to express as fusions to an anchoring phage coat protein and therefore did not display on the phage surface. However, the β-barrel MPs ShuA (Shigella heme uptake A) and MOMP (major outer membrane protein), which pass through the membrane 22 and 16 times, respectively, can display surprisingly well on the surfaces of both conventional and KO7(+) phages. The results provide a guide for protein engineering and large-scale mutagenesis enabled by the phage display of MPs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.