ArticleNature nanotechnology2011
Mixing subattolitre volumes in a quantitative and highly parallel manner with soft matter nanofluidics.
Article in Nature nanotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Programmable RNA Loading of Extracellular Vesicles with Toehold-Release Purification.Journal of the American Chemical Society · 2024Article
- DNA-Programmed Lipid Nanoreactors for Synthesis of Carbohydrate Mimetics by Fusion of Aqueous Sub-attoliter Compartments.Journal of the American Chemical Society · 2023Article
- Single-particle combinatorial multiplexed liposome fusion mediated by DNA.Nature chemistry · 2022Article
- Direct Observation of Sophorolipid Micelle Docking in Model Membranes and Cells by Single Particle Studies Reveals Optimal Fusion Conditions.Biomolecules · 2020Article
- Controlled and Tunable Loading and Release of Vesicles by Using Gigahertz Acoustics.Angewandte Chemie (International ed. in English) · 2019Article
- Guiding protein delivery into live cells using DNA-programmed membrane fusion.Chemical science · 2018Article
- Chemistry in nanoconfined water.Chemical science · 2017Article
- A modular platform for one-step assembly of multi-component membrane systems by fusion of charged proteoliposomes.Nature communications · 2016Article
- Enzymatic reactions in confined environments.Nature nanotechnology · 2016Review
- Shedding light on protein folding, structural and functional dynamics by single molecule studies.Molecules (Basel, Switzerland) · 2014Review
- Geometrical membrane curvature as an allosteric regulator of membrane protein structure and function.Biophysical journal · 2014Article
- Heterogeneous staining: a tool for studies of how fluorescent dyes affect the physical properties of DNA.Nucleic acids research · 2013Article
- Lipid nanotechnology.International journal of molecular sciences · 2013Article
- Femtolitre chemistry assisted by microfluidic pen lithography.Nature communications · 2013Article
- Lipid-based passivation in nanofluidics.Nano letters · 2012Article
- Nanofluidics: Neither shaken nor stirred.Nature nanotechnology · 2011Article
Corrections and comments
- Commented on by
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Handling and mixing ultrasmall volumes of reactants in parallel can increase the throughput and complexity of screening assays while simultaneously reducing reagent consumption. Microfabricated silicon and plastic can provide reliable fluidic devices, but cannot typically handle total volumes smaller than ∼1 × 10(-12) l. Self-assembled soft matter nanocontainers can in principle significantly improve miniaturization and biocompatibility, but exploiting their full potential is a challenge due to their small dimensions. Here, we show that small unilamellar lipid vesicles can be used to mix volumes as small as 1 × 10(-19) l in a reproducible and highly parallelized fashion. The self-enclosed nanoreactors are functionalized with lipids of opposite charge to achieve reliable fusion. Single vesicles encapsulating one set of reactants are immobilized on a glass surface and then fused with diffusing vesicles of opposite charge that carry a complementary set of reactants. We find that ∼85% of the ∼1 × 10(6) cm(-2) surface-tethered nanoreactors undergo non-deterministic fusion, which is leakage-free in all cases, and the system allows up to three to four consecutive mixing events per nanoreactor.
Indexed as
Identifiers
22036813What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.