ArticleHuman pathology2012
Costaining for keratins 8/18 plus ubiquitin improves detection of hepatocyte injury in nonalcoholic fatty liver disease.
Article in Human pathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 74 citations in OpenAlex.
- [Current research progress status in identifying hepatocellular ballooning degeneration in nonalcoholic steatohepatitis].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2025Review
- Digital pathology for nonalcoholic steatohepatitis assessment.Nature reviews. Gastroenterology & hepatology · 2024Review
- Non-alcoholic fatty liver disease: the pathologist's perspective.Clinical and molecular hepatology · 2023Review
- A new preclinical model of western diet-induced progression of non-alcoholic steatohepatitis to hepatocellular carcinoma.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Pathological findings of liver steatosis that is difficult to evaluate with ultrasound.Journal of medical ultrasonics (2001) · 2021Review
- High frequency and long persistency of ballooning hepatocyte were associated with glucose intolerance in patients with severe obesity.Scientific reports · 2021Article
- DNA Methylation in Nonalcoholic Fatty Liver Disease.International journal of molecular sciences · 2020Review
- Automated computerized image analysis for the user-independent evaluation of disease severity in preclinical models of NAFLD/NASH.Laboratory investigation; a journal of technical methods and pathology · 2020Article
- Validity of biopsy-based drug effects in a diet-induced obese mouse model of biopsy-confirmed NASH.BMC gastroenterology · 2019Article
- Review
- Dietary wheat amylase trypsin inhibitors promote features of murine non-alcoholic fatty liver disease.Scientific reports · 2019Article
- Hepatoprotective Effect of Kombucha Tea in Rodent Model of Nonalcoholic Fatty Liver Disease/Nonalcoholic Steatohepatitis.International journal of molecular sciences · 2019Article
- Experimental non-alcoholic steatohepatitis in Göttingen Minipigs: consequences of high fat-fructose-cholesterol diet and diabetes.Journal of translational medicine · 2019Article
- Hedgehog signalling in liver pathophysiology.Journal of hepatology · 2018Review
- A diet-induced animal model of non-alcoholic fatty liver disease and hepatocellular cancer.Journal of hepatology · 2016Article
- Histopathology of nonalcoholic fatty liver disease and nonalcoholic steatohepatitis.Metabolism: clinical and experimental · 2016Review
- Acidophil bodies in nonalcoholic steatohepatitis.Human pathology · 2016Article
- Histology of Nonalcoholic Fatty Liver Disease and Nonalcoholic Steatohepatitis in Adults and Children.Clinics in liver disease · 2016Review
- Histological Assessment of NAFLD.Digestive diseases and sciences · 2016Review
- Characterization of lipid metabolism in a novel immortalized human hepatocyte cell line.American journal of physiology. Endocrinology and metabolism · 2015Article
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Authors and funding
12 authors at 5 institutions in 1 country.
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Abstract
Nonalcoholic fatty liver disease is a global health dilemma. The gold standard for diagnosis is liver biopsy. Ballooned hepatocytes are histologic manifestations of hepatocellular injury and are characteristic of steatohepatitis, the more severe form of nonalcoholic fatty liver disease. Definitive histologic identification of ballooned hepatocytes on routine stains, however, can be difficult. Immunohistochemical evidence for loss of the normal hepatocytic keratin 8/18 can serve as an objective marker of ballooned hepatocytes. We sought to explore the utility of a keratin 8/18 plus ubiquitin double immunohistochemical stain for the histologic evaluation of adult nonalcoholic fatty liver disease. Double immunohistochemical staining for keratin 8/18 and ubiquitin was analyzed using 40 adult human nonalcoholic fatty liver disease core liver biopsies. Ballooned hepatocytes lack keratin 8/18 staining as previously shown by others, but normal-size hepatocytes with keratin loss are approximately 5 times greater in number than keratin-negative ballooned hepatocytes. Keratin-negative ballooned hepatocytes, normal-size hepatocytes with keratin loss, and ubiquitin deposits show a zonal distribution, are positively associated with each other, and are frequently found adjacent to or intermixed with fibrous matrix. All 3 lesions correlate with fibrosis stage and the hematoxylin and eosin diagnosis of steatohepatitis (all P < .05). Compared with hematoxylin and eosin staining, immunohistochemical staining improves the receiver operating characteristics curve for advanced fibrosis (0.77 versus 0.83, 0.89, and 0.89 for keratin-negative ballooned hepatocytes, normal-size hepatocytes with keratin loss, and ubiquitin, respectively) because immunohistochemistry is more sensitive and specific for fibrogenic hepatocellular injury than hematoxylin and eosin staining. Keratin 8/18 plus ubiquitin double immunohistochemical stain improves detection of hepatocyte injury in nonalcoholic fatty liver disease. Thus, it may help differentiate nonalcoholic steatohepatitis from nonalcoholic fatty liver.
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