ArticleJournal of periodontal research2012
Porphyromonas gingivalis promotes monocyte migration by activating MMP-9.
Article in Journal of periodontal research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Exploring the role of oral bacteria in oral cancer: a narrative review.Discover oncology · 2025Review
- Prior vaccination prevents overactivation of innate immune responses during COVID-19 breakthrough infection.Science translational medicine · 2025Article
- Tobacco-enhanced biofilm formation by Porphyromonas gingivalis and other oral microbes.Molecular oral microbiology · 2024Review
- Periodontal status and mandibular biomechanics in rats subjected to hyposalivation and periodontitis.Acta odontologica latinoamericana : AOL · 2024Article
- TNFα induces matrix metalloproteinase-9 expression in monocytic cells through ACSL1/JNK/ERK/NF-kB signaling pathways.Scientific reports · 2023Article
- Porphyromonas gingivalis Gingipains Destroy the Vascular Barrier and Reduce CD99 and CD99L2 Expression To Regulate Transendothelial Migration.Microbiology spectrum · 2023Article
- Metagenomic Analysis Reveals a Mitigating Role forNutrients · 2022Article
- MMP9 Expression Correlates With Cisplatin Resistance in Small Cell Lung Cancer Patients.Frontiers in pharmacology · 2022Article
- Profiles of inflammation factors and inflammatory pathways around the peri-miniscrew implant.Histology and histopathology · 2021Review
- Influence of Gestational Hormones on the Bacteria-Induced Cytokine Response in Periodontitis.Mediators of inflammation · 2021Review
- MMP9 and IGFBP1 Regulate Tumor Immune and Drive Tumor Progression in Clear Cell Renal Cell Carcinoma.Journal of Cancer · 2021Article
- LOX-1 RegulatesFrontiers in cell and developmental biology · 2020Article
- Infection of microglia with Porphyromonas gingivalis promotes cell migration and an inflammatory response through the gingipain-mediated activation of protease-activated receptor-2 in mice.Scientific reports · 2017Article
- Porphyromonas gingivalis promotes invasion of oral squamous cell carcinoma through induction of proMMP9 and its activation.Cellular microbiology · 2014Article
- Neutrophils alter epithelial response to Porphyromonas gingivalis in a gingival crevice model.Molecular oral microbiology · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
BACKGROUND AND
objectiveThe migration of monocytes into the local environment is crucial for their maturation into macrophages or osteoclasts in the pathogenesis of periodontal disease. The objective of this study was to investigate the role and mechanisms mediated by Porphyromonas gingivalis in promoting the migration of monocytes by regulating MMP-9 and TIMP-1 expression. MATERIAL AND
methodsHuman THP1 monocytes were treated with culture supernatant derived from P. gingivalis (ATCC 33277) for 24 h. Zymography, western blot analysis and quantitative PCRs were performed to analyse protein and mRNA levels of MMP-9. Protein and mRNA levels of TIMP-1 from monocytes treated with or without P. gingivalis were determined as well. Transwell migration assay was carried out to analyse the effect of P. gingivalis on the migration of human peripheral blood CD14-positive monocytes. An MMP inhibitor (GM6001) and a proteinase inhibitor (leupeptin) were used to determine the role of MMP-9 in P. gingivalis supernatant- and lipopolysaccharide-induced monocyte migration.
resultsIn zymography and western blot, an 82 kDa band of active MMP-9 emerged in P. gingivalis-treated monocyte culture media in a dose-dependent manner, in addition to the MMP-9 proenzyme (92 kDa) band expressed in control cell culture media. P. gingivalis supernatant increased both the protein and the mRNA levels of MMP-9 and TIMP-1. P. gingivalis supernatant, but not its lipopolysaccharide, increased the migratory ability of CD14-positive monocytes. The increased migratory ability of P. gingivalis-treated monocytes was partly inhibited by leupeptin (200 μg/mL) and completely antagonized by the MMP inhibitor GM6001 (100 nm). Lipopolysaccharide of P. gingivalis increased protein and mRNA levels of MMP-9 in monocytes, but had no effect on the migratory ability or MMP-9 activation.
conclusionP. gingivalis supernatant increased the migratory ability of monocytes, in part, by increasing activation and expression of MMP-9.
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