ArticleStem cells and development2012
Osteogenic differentiation of stem cells alters vitamin D receptor expression.
Article in Stem cells and development, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Vitamin D Enhanced the Osteogenic Differentiation of Cell Spheroids Composed of Bone Marrow Stem Cells.Medicina (Kaunas, Lithuania) · 2021Article
- Placental proteome abnormalities in women with gestational diabetes and large-for-gestational-age newborns.BMJ open diabetes research & care · 2020Article
- Vitamin D history part III: the "modern times"-new questions for orthopaedic practice: deficiency, cell therapy, osteomalacia, fractures, supplementation, infections.International orthopaedics · 2019Review
- Multifunctional molecule ERp57: From cancer to neurodegenerative diseases.Pharmacology & therapeutics · 2018Review
- Genome‑wide analysis and prediction of functional long noncoding RNAs in osteoblast differentiation under simulated microgravity.Molecular medicine reports · 2017Article
- (+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor.Experimental and therapeutic medicine · 2017Article
- MiR-351 negatively regulates osteoblast differentiation of MSCs induced by (+)-cholesten-3-one through targeting VDR.American journal of translational research · 2017Article
- Fibromodulin reprogrammed cells: A novel cell source for bone regeneration.Biomaterials · 2016Article
- Article
- Osteogenic embryoid body-derived material induces bone formation in vivo.Scientific reports · 2015Article
- Phosphatidylethanolamine biomimetic coating increases mesenchymal stem cell osteoblastogenesis.Journal of materials science. Materials in medicine · 2014Article
- Impaired bone formation in Pdia3 deficient mice.PloS one · 2014Article
- Chaperone properties of pdia3 participate in rapid membrane actions of 1α,25-dihydroxyvitamin d3.Molecular endocrinology (Baltimore, Md.) · 2013Article
- Osteoblast maturation and new bone formation in response to titanium implant surface features are reduced with age.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2012Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Pluripotent and multipotent stem cells adopt an osteoblastic phenotype when cultured in environments that enhance their osteogenic potential. Embryonic stem cells differentiated as embryoid bodies (EBs) in osteogenic medium containing β-glycerophosphate exhibit increased expression of bone markers, indicating that cells are osteoblastic. Interestingly, 1α,25-dihydroxyvitaminD3 (1,25D) enhances the osteogenic phenotype not just in EBs but also in multipotent adult mesenchymal stem cells (MSCs). 1,25D acts on osteoblasts via classical vitamin D receptors (VDR) and via a membrane 1,25D-binding protein [protein disulfide isomerase family A, member 3 (PDIA3)], which activates protein kinase C-signaling. The aims of this study were to determine whether these receptors are regulated during osteogenic differentiation of stem cells and if stem cells and differentiated progeny are responsive to 1,25D. mRNA and protein levels for VDR, PDIA3, and osteoblast-associated proteins were measured in undifferentiated cells and in cells treated with osteogenic medium. Mouse EBs expressed both VDR and PDIA3, but VDR increased as cells underwent osteogenic differentiation. Human MSCs expressed Pdia3 at constant levels throughout differentiation, but VDR increased in cells treated with osteogenic medium. These results suggest that both 1,25D signaling mechanisms are important, with PDIA3 playing a greater role during early events and VDR playing a greater role in later stages of differentiation. Understanding these coordinated events provide a powerful tool to control pluripotent and multipotent stem cell differentiation through induction medium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.