ArticleBioconjugate chemistry2011
Multi-path quenchers: efficient quenching of common fluorophores.
Article in Bioconjugate chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Chemical Synthesis of Mirror-Image Proteins Reveals Chirality-Dependent Cellular Uptake Mediated by a Cell-Penetrating Peptide.Journal of the American Chemical Society · 2026Article
- Characterization of Fluorophore-Quencher Pairs for Distance-Dependent Molecular Sensing in Photoacoustic Imaging.Chemical & biomedical imaging · 2026Article
- Fluorescent modifications in aptamer switches-positional, structural, and neighboring pair effects on sensor performance.Nucleic acids research · 2025Article
- Interactions of Ligand, Aptamer, and Complementary Oligonucleotide: Studying Impacts of NaMolecules (Basel, Switzerland) · 2025Article
- Y-shaped DNA as a dynamic self-assembly nanomaterial for phenotype-specific regulation of stem cell differentiation on the gene level.Regenerative biomaterials · 2025Article
- The potential of fluorogenicity for single molecule FRET and DyeCycling.QRB discovery · 2024Review
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- Stimuli-responsive azobenzene-quantum dots for multi-sensing of dithionite, hypochlorite, and azoreductase.Mikrochimica acta · 2020Article
- A "Quenchergenic" Chemoselective Protein Labeling Strategy.Chembiochem : a European journal of chemical biology · 2019Article
- Imaging Integrin Tension and Cellular Force at Submicron Resolution with an Integrative Tension Sensor.Journal of visualized experiments : JoVE · 2019Article
- Novel tool to quantify cell wall porosity relates wall structure to cell growth and drug uptake.The Journal of cell biology · 2019Article
- Rapid and sensitive detection of the activity of ADAM17 using a graphene oxide-based fluorescence sensor.The Analyst · 2019Article
- Keratocytes Generate High Integrin Tension at the Trailing Edge to Mediate Rear De-adhesion during Rapid Cell Migration.iScience · 2018Article
- Multiplex qPCR for serodetection and serotyping of hepatitis viruses: A brief review.World journal of gastroenterology · 2016Review
- A broad spectrum dark quencher: construction of multiple colour protease and photolytic sensors.Chemical communications (Cambridge, England) · 2013Article
- Fluorescence quenchers for hydrazone and oxime orthogonal bioconjugation.Bioconjugate chemistry · 2012Article
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Authors and funding
2 authors.
Funding
Abstract
Fluorescence quenching groups are widely employed in biological detection, sensing, and imaging. To date, a relatively small number of such groups are in common use. Perhaps the most commonly used quencher, dabcyl, has limited efficiency with a broad range of fluorophores. Here, we describe a molecular approach to improve the efficiency of quenchers by increasing their electronic complexity. Multi-Path Quenchers (MPQ) are designed to have multiple donor or acceptor groups in their structure, allowing for a multiplicity of conjugation pathways of varied length. This has the effect of broadening the absorption spectrum, which in turn can increase quenching efficiency and versatility. Six such MPQ derivatives are synthesized and tested for quenching efficiency in a DNA hybridization context. Duplexes placing quenchers and fluorophores within contact distance or beyond this distance are used to measure quenching via contact or FRET mechanisms. Results show that several of the quenchers are considerably more efficient than dabcyl at quenching a wider range of common fluorophores, and two quench fluorescein and TAMRA as well as or better than a Black Hole Quencher.
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