ArticlePlant physiology2011
Laser microdissection unravels cell-type-specific transcription in arbuscular mycorrhizal roots, including CAAT-box transcription factor gene expression correlating with fungal contact and spread.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed.
- Integrating single-cell omic techniques to resolve the spatio-temporal complexity of arbuscular mycorrhizal symbiosis.Journal of experimental botany · 2026Review
- Transcriptomic Profiling Identifies Symbiosis-Induced GDSL Lipase Genes Associated with Soybean-Arbuscular Mycorrhizal Symbiosis.International journal of molecular sciences · 2026Article
- Co-extraction of Small RNAs and Total RNA from Arbuscular Mycorrhizal Roots Using Laser Microdissection.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A transcriptomic perspective of P trade in mycorrhizal grapevine.Mycorrhiza · 2025Article
- Genome-Wide Identification and Expression Pattern Analysis of Nuclear Factor Y B/C Genes inPlants (Basel, Switzerland) · 2025Article
- Article
- Breaking barriers: improving time and space resolution of arbuscular mycorrhizal symbiosis with single-cell sequencing approaches.Biology direct · 2024Review
- Phosphatidyl Ethanolamine Binding ProteinInternational journal of molecular sciences · 2024Article
- Understanding the root of the problem for tackling pea root rot disease.Frontiers in microbiology · 2024Review
- How to explore what is hidden? A review of techniques for vascular tissue expression profile analysis.Plant methods · 2023Review
- Signaling and Detoxification Strategies in Plant-Microbes Symbiosis under Heavy Metal Stress: A Mechanistic Understanding.Microorganisms · 2022Review
- The Roles of Phosphorus and Nitrogen Nutrient Transporters in the Arbuscular Mycorrhizal Symbiosis.International journal of molecular sciences · 2022Review
- Mycorrhizal symbiosis reprograms ion fluxes and fatty acid metabolism in wild jujube during salt stress.Plant physiology · 2022Article
- The potential role of Mucoromycotina 'fine root endophytes' in plant nitrogen nutrition.Physiologia plantarum · 2022Article
- MtNF-YC6 and MtNF-YC11 are involved in regulating the transcriptional program of arbuscular mycorrhizal symbiosis.Frontiers in plant science · 2022Article
- NF-YB family transcription factors inFrontiers in microbiology · 2022Article
- Plant-Mycorrhizal Fungi Interactions in Phytoremediation of Geogenic Contaminated Soils.Frontiers in microbiology · 2022Review
- A roadmap of plant membrane transporters in arbuscular mycorrhizal and legume-rhizobium symbioses.Plant physiology · 2021Review
- A Transcriptomic Approach Provides Insights on the Mycorrhizal Symbiosis of the Mediterranean OrchidPlants (Basel, Switzerland) · 2021Article
- Photosynthetic Traits and Nitrogen Uptake in Crops: Which Is the Role of Arbuscular Mycorrhizal Fungi?Plants (Basel, Switzerland) · 2020Review
22 more citing papers are in PubMed but not listed here.
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6 authors.
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Abstract
Arbuscular mycorrhizae (AM) are the most widespread symbioses on Earth, promoting nutrient supply of most terrestrial plant species. To unravel gene expression in defined stages of Medicago truncatula root colonization by AM fungi, we here combined genome-wide transcriptome profiling based on whole mycorrhizal roots with real-time reverse transcription-PCR experiments that relied on characteristic cell types obtained via laser microdissection. Our genome-wide approach delivered a core set of 512 genes significantly activated by the two mycorrhizal fungi Glomus intraradices and Glomus mossae. Focusing on 62 of these genes being related to membrane transport, signaling, and transcriptional regulation, we distinguished whether they are activated in arbuscule-containing or the neighboring cortical cells harboring fungal hyphae. In addition, cortical cells from nonmycorrhizal roots served as a reference for gene expression under noncolonized conditions. Our analysis identified 25 novel arbuscule-specific genes and 37 genes expressed both in the arbuscule-containing and the adjacent cortical cells colonized by fungal hyphae. Among the AM-induced genes specifying transcriptional regulators were two members encoding CAAT-box binding transcription factors (CBFs), designated MtCbf1 and MtCbf2. Promoter analyses demonstrated that both genes were already activated by the first physical contact between the symbionts. Subsequently, and corresponding to our cell-type expression patterns, they were progressively up-regulated in those cortical areas colonized by fungal hyphae, including the arbuscule-containing cells. The encoded CBFs thus represent excellent candidates for regulators that mediate a sequential reprogramming of root tissues during the establishment of an AM symbiosis.
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