ArticleJournal of virology2012
Modification of the untranslated regions of human enterovirus 71 impairs growth in a cell-specific manner.
Article in Journal of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Enterovirus A71 priorities, challenges, and future opportunities in humoral immunity and vaccine development.NPJ vaccines · 2025Review
- Insight into the Life Cycle of Enterovirus-A71.Viruses · 2025Review
- Review
- The Stem-Loop I of Senecavirus A IRES Is Essential for Cap-Independent Translation Activity and Virus Recovery.Viruses · 2021Article
- Translation control of Enterovirus A71 gene expression.Journal of biomedical science · 2020Review
- Article
- Article
- The Roles of Picornavirus Untranslated Regions in Infection and Innate Immunity.Frontiers in microbiology · 2018Review
- Enterovirus A71 Proteins: Structure and Function.Frontiers in microbiology · 2018Review
- Modification of the internal ribosome entry site element impairs the growth of foot-and-mouth disease virus in porcine-derived cells.The Journal of general virology · 2016Article
- Therapeutic and prevention strategies against human enterovirus 71 infection.World journal of virology · 2015Review
- Inhibition of enterovirus 71 by adenosine analog NITD008.Journal of virology · 2014Article
- Cell and tissue tropism of enterovirus 71 and other enteroviruses infections.Journal of biomedical science · 2014Review
- Role of RNA structure motifs in IRES-dependent translation initiation of the coxsackievirus B3: new insights for developing live-attenuated strains for vaccines and gene therapy.Molecular biotechnology · 2013Review
- Molecular Analysis of RNA-RNA Interactions between 5' and 3' Untranslated Regions during the Initiation of Translation of a Cardiovirulent and a Live-Attenuated Coxsackievirus B3 Strains.International journal of molecular sciences · 2013Article
- The Pathogenesis and Prevention of Encephalitis due to Human Enterovirus 71.Current infectious disease reports · 2012Article
- Identification of site-specific adaptations conferring increased neural cell tropism during human enterovirus 71 infection.PLoS pathogens · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human enterovirus 71 (HEV71) is the causative agent of hand, foot, and mouth disease and associated acute neurological disease. At present, little is known about the genetic determinants of HEV71 neurovirulence. Studies of related enteroviruses have indicated that the untranslated regions (UTRs), which control virus-directed translation and replication, also exert significant influence on neurovirulence. We used an infectious cDNA clone of a subgenogroup B3 strain to construct and characterize chimeras with 5'- and 3'-UTR modifications. Replacement of the entire HEV71 5' UTR with that of human rhinovirus 2 (HRV2) resulted in a small reduction in growth efficiency in cells of both nonneuronal (rhabdomyosarcoma) and neuronal (SH-SY5Y) origin due to reduced translational efficiency. However, the introduction of a 17-nucleotide deletion into the proximal region of the 3' UTR significantly decreased the growth of HEV71-HRV2 in SH-SY5Y cells. This observation is similar to that made with stem-loop domain Z (SLD Z)-deleted coxsackievirus B3-HRV2 5'-UTR chimeras reported previously and provides the first evidence of a potentially functional SLD Z in the 3' UTR in human enterovirus A species viruses. We further showed that the cell-specific growth impairment was caused by the synergistic effects of cis-acting UTR control elements on different stages of the virus life cycle. These chimeras will further improve our understanding of the control of HEV71 replication and its relationship to neurovirulence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.