ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2011
Early emergence of neural activity in the developing mouse enteric nervous system.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Functional maturation of enteric neurons derived from human induced pluripotent stem cells.Stem cell reports · 2026Article
- Spontaneous enteric nervous system activity generates contractile patterns prior to maturation of gastrointestinal motility.Neurogastroenterology and motility · 2026Article
- Tetrodotoxin-resistant mechanosensitivity and L-type calcium channel-mediated spontaneous calcium activity in enteric neurons.Experimental physiology · 2024Article
- Sequencing Reveals miRNAs Enriched in the Developing Mouse Enteric Nervous System.Non-coding RNA · 2023Article
- Functional and Transcriptomic Characterization of Postnatal Maturation of ENS and SIP Syncytium in Mice Colon.Biomolecules · 2023Article
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- A functional network of highly pure enteric neurons in a dish.Frontiers in neuroscience · 2022Article
- Isolation and transfection of myenteric neurons from mice for patch-clamp applications.Frontiers in molecular neuroscience · 2022Article
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- Muscularis macrophage development in the absence of an enteric nervous system.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Optogenetic and chemogenetic techniques for neurogastroenterology.Nature reviews. Gastroenterology & hepatology · 2018Review
- VPAC Receptor Subtypes Tune Purinergic Neuron-to-Glia Communication in the Murine Submucosal Plexus.Frontiers in cellular neuroscience · 2017Article
- White paper on guidelines concerning enteric nervous system stem cell therapy for enteric neuropathies.Developmental biology · 2016Review
- Simultaneous optical and electrical in vivo analysis of the enteric nervous system.Nature communications · 2016Article
- In Vivo Transplantation of Enteric Neural Crest Cells into Mouse Gut; Engraftment, Functional Integration and Long-Term Safety.PloS one · 2016Article
- Optical Tools to Investigate Cellular Activity in the Intestinal Wall.Journal of neurogastroenterology and motility · 2015Article
- Changes in Nicotinic Neurotransmission during Enteric Nervous System Development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Building a second brain in the bowel.The Journal of clinical investigation · 2015Review
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurons of the enteric nervous system (ENS) arise from neural crest cells that migrate into and along the developing gastrointestinal tract. A subpopulation of these neural-crest derived cells express pan-neuronal markers early in development, shortly after they first enter the gut. However, it is unknown whether these early enteric "neurons" are electrically active. In this study we used live Ca(2+) imaging to examine the activity of enteric neurons from mice at embryonic day 11.5 (E11.5), E12.5, E15.5, and E18.5 that were dissociated and cultured overnight. PGP9.5-immunoreactive neurons from E11.5 gut cultures responded to electrical field stimulation with fast [Ca(2+)](i) transients that were sensitive to TTX and ω-conotoxin GVIA, suggesting roles for voltage-gated Na(+) channels and N-type voltage-gated Ca(2+) channels. E11.5 neurons were also responsive to the nicotinic cholinergic agonist, dimethylphenylpiperazinium, and to ATP. In addition, spontaneous [Ca(2+)](i) transients were present. Similar responses were observed in neurons from older embryonic gut. Whole-cell patch-clamp recordings performed on E12.5 enteric neurons after 2-10 h in culture revealed that these neurons fired both spontaneous and evoked action potentials. Together, our results show that enteric neurons exhibit mature forms of activity at early stages of ENS development. This is the first investigation to directly examine the presence of neural activity during enteric neuron development. Along with the spinal cord and hindbrain, the ENS appears to be one of the earliest parts of the nervous system to exhibit electrical activity.
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