ArticleJournal of clinical microbiology2012
Loop-mediated isothermal amplification assays for detecting shiga toxin-producing Escherichia coli in ground beef and human stools.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 113 citations in OpenAlex.
- Development of Loop-Mediated Isothermal Amplification Assay for the Detection of aaic Positive Enteroaggregative Escherichia coli (EAEC).Applied biochemistry and biotechnology · 2026Article
- Development loop-mediated isothermal amplification assay for detection of enteropathogenic Escherichia coli (EPEC).Molecular biology reports · 2026Article
- Naked Eye Meets Nucleic Acids: A Review on Dyes for Isothermal Amplification Methods for Detection of Meat Adulteration.Journal of fluorescence · 2026Review
- Comparative evaluation of 14Frontiers in microbiology · 2026Article
- Development of loop-mediated isothermal amplification (LAMP) assays for the detection of diarrheagenicApplied and environmental microbiology · 2025Article
- Article
- Evaluation and Optimization of Loop-Mediated Isothermal Amplification (LAMP) Technique for Capripoxvirus Diagnosis and Its Comparison with PCR Method.Archives of Razi Institute · 2024Article
- Colorimetric and electrochemical analysis of DNAzyme-LAMP amplicons for the detection of Escherichia coli in food matrices.Scientific reports · 2024Article
- Integrating loop-mediated isothermal amplification with lateral flow assay to achieve a highly sensitive method for detectingHeliyon · 2024Article
- A rapid on-site loop-mediated isothermal amplification technology as an early warning system for the detection of Shiga toxin-producingMicrobiology (Reading, England) · 2024Article
- Rapid and visual detection of Shiga-toxigenic Escherichia coli (STEC) in carabeef meat harnessing loop-mediated isothermal amplification (LAMP).Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Article
- Diagnosis of extraintestinal pathogenicCurrent research in microbial sciences · 2024Review
- Loop mediated isothermal amplification as a molecular diagnostic assay: Application and evaluation for detection of EnterohaemorrhagicPractical laboratory medicine · 2023Article
- Rapid detection of Shiga-toxin-producing Escherichia coli O157:H7 based on a colorimetric loop-mediated isothermal amplification (cLAMP) assay using a molecular beacon paired with HRPzyme.Analytical and bioanalytical chemistry · 2023Article
- A new multiplex qPCR assay to detect and differentiate big cat species in the illegal wildlife trade.Scientific reports · 2023Article
- Recent Advancements in Technologies to Detect EnterohaemorrhagicJournal of microbiology and biotechnology · 2023Review
- Virus Detection: From State-of-the-Art Laboratories to Smartphone-Based Point-of-Care Testing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification and PCR Methods in Detection of Foodborne Microorganisms: A Systematic Review and Meta-Analysis.Iranian journal of public health · 2021Review
- Viable but Nonculturable Escherichia coli O157:H7 and Salmonella enterica in Fresh Produce: Rapid Determination by Loop-Mediated Isothermal Amplification Coupled with a Propidium Monoazide Treatment.Applied and environmental microbiology · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shiga toxin-producing Escherichia coli (STEC), encompassing E. coli O157 and non-O157 STEC, is a significant cause of food-borne illnesses and deaths in the United States and worldwide. Shiga toxins (encoded by stx) and intimin (encoded by eae) are important virulence factors for STEC strains linked to severe human illnesses such as hemorrhagic colitis and hemolytic-uremic syndrome. In this study, the stx(1), stx(2), and eae genes were chosen as targets to design loop-mediated isothermal amplification (LAMP) assays for the rapid, specific, sensitive, and quantitative detection of STEC strains. The assay performances in pure culture and spiked ground beef and human stools were evaluated and compared with those of quantitative PCR (qPCR). No false-positive or false-negative results were observed among 90 bacterial strains used to evaluate assay specificity. The limits of detection for seven STEC strains of various serogroups (O26, O45, O103, O111, O121, O145, and O157) were approximately 1 to 20 CFU/reaction in pure culture and 10(3) to 10(4) CFU/g in spiked ground beef, which were comparable to the results of qPCR. Standard curves generated suggested good linear relationships between STEC cell numbers and LAMP turbidity signals. When applied in ground beef samples spiked with two low levels (1 to 2 and 10 to 20 CFU/25 g) of STEC cultures, the LAMP assays achieved accurate detection after 6 to 8 h enrichment. The assays also consistently detected STEC in human stool specimens spiked with 10(3) or 10(4) CFU/0.5 g stool after 4 h enrichment, while qPCR required 4 to 6 h. In conclusion, the LAMP assays developed in this study may facilitate rapid and reliable identification of STEC contaminations in high-risk food commodities and also facilitate prompt diagnosis of STEC infections in clinical laboratories.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.