ArticleProceedings of the National Academy of Sciences of the United States of America2011
Three-dimensional superresolution colocalization of intracellular protein superstructures and the cell surface in live Caulobacter crescentus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 146 citations in OpenAlex.
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- Phospho-signaling couples polar asymmetry and proteolysis within a membraneless microdomain in Caulobacter crescentus.Nature communications · 2024Article
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- Filament structure and subcellular organization of the bacterial intermediate filament-like protein crescentin.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Super-resolution microscopy to study membrane nanodomains and transport mechanisms in the plasma membrane.Frontiers in molecular biosciences · 2024Review
- Imaging and positioning through scattering media with double-helix point spread function engineering.Journal of biomedical optics · 2023Article
- Nanoscopy for endosomal escape quantification.Nanoscale advances · 2021Review
- Towards a Quantitative Single Particle Characterization by Super Resolution Microscopy: From Virus Structures to Antivirals Design.Frontiers in bioengineering and biotechnology · 2021Review
- The composition and function ofmicroLife · 2021Article
- Single-molecule localization microscopy.Nature reviews. Methods primers · 2021Article
- Probing Nanoscale Diffusional Heterogeneities in Cellular Membranes through Multidimensional Single-Molecule and Super-Resolution Microscopy.Journal of the American Chemical Society · 2020Article
- Strategies to maximize performance in STimulated Emission Depletion (STED) nanoscopy of biological specimens.Methods (San Diego, Calif.) · 2020Review
- Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells.Methods (San Diego, Calif.) · 2020Article
- Microgel PAINT - nanoscopic polarity imaging of adaptive microgels without covalent labelling.Chemical science · 2019Article
- Enabling technologies in super-resolution fluorescence microscopy: reporters, labeling, and methods of measurement.Current opinion in structural biology · 2019Review
- 3D Tracking-Free Approach for Obtaining 3D Super-Resolution Information in Rotationally Symmetric Biostructures.The journal of physical chemistry. B · 2019Article
- Revealing Nanoscale Morphology of the Primary Cilium Using Super-Resolution Fluorescence Microscopy.Biophysical journal · 2019Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Recently, single-molecule imaging and photocontrol have enabled superresolution optical microscopy of cellular structures beyond Abbe's diffraction limit, extending the frontier of noninvasive imaging of structures within living cells. However, live-cell superresolution imaging has been challenged by the need to image three-dimensional (3D) structures relative to their biological context, such as the cellular membrane. We have developed a technique, termed superresolution by power-dependent active intermittency and points accumulation for imaging in nanoscale topography (SPRAIPAINT) that combines imaging of intracellular enhanced YFP (eYFP) fusions (SPRAI) with stochastic localization of the cell surface (PAINT) to image two different fluorophores sequentially with only one laser. Simple light-induced blinking of eYFP and collisional flux onto the cell surface by Nile red are used to achieve single-molecule localizations, without any antibody labeling, cell membrane permeabilization, or thiol-oxygen scavenger systems required. Here we demonstrate live-cell 3D superresolution imaging of Crescentin-eYFP, a cytoskeletal fluorescent protein fusion, colocalized with the surface of the bacterium Caulobacter crescentus using a double-helix point spread function microscope. Three-dimensional colocalization of intracellular protein structures and the cell surface with superresolution optical microscopy opens the door for the analysis of protein interactions in living cells with excellent precision (20-40 nm in 3D) over a large field of view (12 12 μm).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.