ArticleOtolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery2012
In vivo implantation of tissue-engineered human nasal septal neocartilage constructs: a pilot study.
Article in Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Clinical Application of 3-Dimensional Printing Technology for Patients With Nasal Septal Deformities: A Multicenter Study.JAMA otolaryngology-- head & neck surgery · 2018Trial
- Auricular Reconstruction Following Traumatic Amputation: A Historical Review and Contemporary Treatment Algorithm.Journal of clinical medicine · 2026Review
- Advancement in Sodium Alginate-Based Drug Delivery Systems: Applications and Future Prospects.AAPS PharmSciTech · 2026Review
- Article
- Human Septal Cartilage Tissue Engineering: Current Methodologies and Future Directions.Bioengineering (Basel, Switzerland) · 2024Review
- Tissue Engineering and Regenerative Medicine in the Field of Otorhinolaryngology.Tissue engineering and regenerative medicine · 2024Review
- Application of Tissue Engineering and Biomaterials in Nose Surgery.JPRAS open · 2024Review
- Tissue engineering in otorhinolaryngology: A knowledge-based analysis.Laryngoscope investigative otolaryngology · 2024Article
- Nuclear Factor-κB Decoy Oligodeoxynucleotide Attenuates Cartilage Resorption In Vitro.Bioengineering (Basel, Switzerland) · 2024Article
- Achievements and Challenges in Transplantation of Mesenchymal Stem Cells in Otorhinolaryngology.Journal of clinical medicine · 2021Review
- Cartilage tissue engineering for craniofacial reconstruction.Archives of plastic surgery · 2020Article
- Neural Crest-Derived Chondrocytes Isolation for Tissue Engineering in Regenerative Medicine.Cells · 2020Article
- Alginate Formulations: Current Developments in the Race for Hydrogel-Based Cardiac Regeneration.Frontiers in bioengineering and biotechnology · 2020Review
- Evaluation of Autogenous Engineered Septal Cartilage Grafts in Rabbits- A Minimally Invasive Preclinical Model.Advances in otolaryngology · 2014Article
- Engineered nasal cartilage by cell homing: a model for augmentative and reconstructive rhinoplasty.Plastic and reconstructive surgery · 2014Article
- Volume Expansion of Tissue Engineered Human Nasal Septal Cartilage.Journal of otology & rhinology · 2014Article
- A compositional analysis of cadaveric human nasal septal cartilage.The Laryngoscope · 2013Article
- Shape fidelity of native and engineered human nasal septal cartilage.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2013Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveTo determine the in vivo biocompatibility of septal neocartilage constructs developed in vitro by an alginate intermediate step. STUDY
designProspective, animal model.
settingResearch laboratory. SUBJECTS AND
methodsA murine model was used to examine the maturation of neocartilage constructs in vivo. Chondrocytes collected from patients undergoing septoplasty were expanded in monolayer and suspended in alginate beads for 3-dimensional culture in media containing human serum and growth factors. After in vitro incubation for 5 weeks, the constructs were implanted in the dorsum of athymic mice for 30 and 60 days (n = 9). After the mice were sacrificed, the constructs were recovered for assessment of their morphological, histochemical, biochemical, and biomechanical properties.
resultsThe mice survived and tolerated the implants well. Infection and extrusion were not observed. Neocartilage constructs maintained their general shape and size and demonstrated cell viability after implantation. The implanted constructs were firm and opaque, sharing closer semblance to native septal tissue relative to the gelatinous, translucent preimplant constructs. Histochemical staining with hematoxylin and eosin (H&E) revealed that the constructs exhibited distinct morphologies characteristic of native tissue, which were not observed in preimplant constructs. DNA and type II collagen increased with duration of implantation, whereas type I collagen and glycoaminoglycans (GAG) decreased. Mechanical testing of a 60-day implanted construct demonstrated characteristics similar to native human septal cartilage.
conclusionsNeocartilage constructs are viable in an in vivo murine model. The histologic, biochemical, and biomechanical features of implanted constructs closely resemble native septal tissue when compared with preimplant constructs.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.