ArticleMolecular biology of the cell2011
Cbk1 kinase and Bck2 control MAP kinase activation and inactivation during heat shock.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Single-cell imaging reveals a key role of Bck2 in yeast cell size adaptation to nutrient challenges.The Journal of cell biology · 2026Article
- A conserved fungal morphogenetic kinase regulates pathogenic growth in response to carbon source diversity.Nature communications · 2024Article
- A novel MAP kinase-interacting protein MoSmi1 regulates development and pathogenicity in Magnaporthe oryzae.Molecular plant pathology · 2024Article
- A walk-through MAPK structure and functionality with the 30-year-old yeast MAPK Slt2.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Review
- Regulation of biotic interactions and responses to abiotic stresses by MAP kinase pathways in plant pathogenic fungi.Stress biology · 2021Review
- MOB: Pivotal Conserved Proteins in Cytokinesis, Cell Architecture and Tissue Homeostasis.Biology · 2020Review
- Ssd1 and the cell wall integrity pathway promote entry, maintenance, and recovery from quiescence in budding yeast.Molecular biology of the cell · 2019Article
- Mitogen-Activated Protein Kinase Phosphatases (MKPs) in Fungal Signaling: Conservation, Function, and Regulation.International journal of molecular sciences · 2019Review
- Heat-stress triggers MAPK crosstalk to turn on the hyperosmotic response pathway.Scientific reports · 2018Article
- Ndr kinases regulate retinal interneuron proliferation and homeostasis.Scientific reports · 2018Article
- The eukaryotic protein kinase superfamily of the necrotrophic fungal plant pathogen, Sclerotinia sclerotiorum.Molecular plant pathology · 2016Review
- The Kinome of Edible and Medicinal FungusFrontiers in microbiology · 2016Article
- Alterations of protein expression in conditions of copper-deprivation for Paracoccidioides lutzii in the presence of extracellular matrix components.BMC microbiology · 2014Article
- Bck2 acts through the MADS box protein Mcm1 to activate cell-cycle-regulated genes in budding yeast.PLoS genetics · 2013Article
- The RAM network in pathogenic fungi.Eukaryotic cell · 2012Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Saccharomyces cerevisiae Cbk1 kinase is a LATS/NDR tumor suppressor orthologue and component of the Regulation of Ace2 and Morphogenesis signaling network. Cbk1 was previously implicated in regulating polarized morphogenesis, gene expression, and cell integrity. Here we establish that Cbk1 is critical for heat shock and cell wall stress signaling via Bck2, a protein associated with the Pkc1-Mpk1 cell integrity pathway. We demonstrate that cbk1 and bck2 loss-of-function mutations prevent Mpk1 kinase activation and Mpk1-dependent gene expression but do not disrupt Mpk1 Thr-190/Tyr-192 phosphorylation. Bck2 overexpression partially restores Mpk1-dependent Rlm1 transcription factor activity in cbk1 mutants, suggesting that Bck2 functions downstream of Cbk1. We demonstrate that Bck2 precisely colocalizes with the mitogen-activated protein kinase (MAPK) phosphatase Sdp1. During heat shock, Bck2 and Sdp1 transiently redistribute from nuclei and the cytosol to mitochondria and other cytoplasmic puncta before returning to their pre-stressed localization patterns. Significantly, Cbk1 inhibition delays the return of Bck2 and Sdp1 to their pre-stressed localization patterns and delays Mpk1 Thr-190/Tyr-192 dephosphorylation upon heat shock adaptation. We conclude that Cbk1 and Bck2 are required for Mpk1 activation during heat shock and cell wall stress and for Mpk1 dephosphorylation during heat shock adaptation. These data provide the first evidence that Cbk1 kinase regulates MAPK-dependent stress signaling and provide mechanistic insight into Sdp1 phosphatase regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.