ArticleCell cycle (Georgetown, Tex.)2011
Differential epithelium DNA damage response to ATM and DNA-PK pathway inhibition in human prostate tissue culture.
Article in Cell cycle (Georgetown, Tex.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Excess growth hormone suppresses DNA damage repair in epithelial cells.JCI insight · 2019Article
- ELL2 regulates DNA non-homologous end joining (NHEJ) repair in prostate cancer cells.Cancer letters · 2018Article
- Novel Assay to Detect RNA Polymerase I ActivityMolecular cancer research : MCR · 2017Article
- EAF2 regulates DNA repair through Ku70/Ku80 in the prostate.Oncogene · 2017Article
- Evaluation of surrogate tissues as indicators of drug activity in a melanoma skin model.Cancer medicine · 2016Article
- Small molecule BMH-compounds that inhibit RNA polymerase I and cause nucleolar stress.Molecular cancer therapeutics · 2014Article
- DNA intercalator BMH-21 inhibits RNA polymerase I independent of DNA damage response.Oncotarget · 2014Article
- DNA damage signaling regulates age-dependent proliferative capacity of quiescent inner ear supporting cells.Aging · 2014Article
- A tissue graft model of DNA damage response in the normal and malignant human prostate.The Journal of urology · 2014Article
- p53 shapes genome-wide and cell type-specific changes in microRNA expression during the human DNA damage response.Cell cycle (Georgetown, Tex.) · 2014Article
- HDAC inhibitor confers radiosensitivity to prostate stem-like cells.British journal of cancer · 2013Article
- Regulatory mechanisms and clinical perspectives of miRNA in tumor radiosensitivity.Carcinogenesis · 2012Review
- Maintenance of genomic integrity after DNA double strand breaks in the human prostate and seminal vesicle epithelium: the best and the worst.Molecular oncology · 2012Review
- DNA damage responses in the prostate: implications for cancer formation?Cell cycle (Georgetown, Tex.) · 2011Article
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9 authors at 6 institutions in 3 countries.
Funding
Abstract
The ability of cells to respond and repair DNA damage is fundamental for the maintenance of genomic integrity. Ex vivo culturing of surgery-derived human tissues has provided a significant advancement to assess DNA damage response (DDR) in the context of normal cytoarchitecture in a non-proliferating tissue. Here, we assess the dependency of prostate epithelium DDR on ATM and DNA-PKcs, the major kinases responsible for damage detection and repair by nonhomologous end-joining (NHEJ), respectively. DNA damage was caused by ionizing radiation (IR) and cytotoxic drugs, cultured tissues were treated with ATM and DNA-PK inhibitors, and DDR was assessed by phosphorylation of ATM and its targets H2AX and KAP1, a heterochromatin binding protein. Phosphorylation of H2AX and KAP1 was fast, transient and fully dependent on ATM, but these responses were moderate in luminal cells. In contrast, DNA-PKcs was phosphorylated in both luminal and basal cells, suggesting that DNA-PK-dependent repair was also activated in the luminal cells despite the diminished H2AX and KAP1 responses. These results indicate that prostate epithelial cell types have constitutively dissimilar responses to DNA damage. We correlate the altered damage response to the differential chromatin state of the cells. These findings are relevant in understanding how the epithelium senses and responds to DNA damage.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.