ArticlePloS one2011
Microenvironmental geometry guides platelet adhesion and spreading: a quantitative analysis at the single cell level.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- Effect of two-dimensional spatial confinement on platelet mechanics.Biophysical reports · 2026Article
- A Synergistic Overview between Microfluidics and Numerical Research for Vascular Flow and Pathological Investigations.Sensors (Basel, Switzerland) · 2024Review
- Why platelet mechanotransduction matters for hemostasis and thrombosis.Journal of thrombosis and haemostasis : JTH · 2023Review
- Evaluation of focal adhesion mediated subcellular curvature sensing in response to engineered extracellular matrix.Biointerphases · 2023Article
- Article
- Getting a good view:Platelets · 2020Review
- Statistical analysis of 3D localisation microscopy images for quantification of membrane protein distributions in a platelet clot model.PLoS computational biology · 2020Article
- The biophysics and mechanics of blood from a materials perspective.Nature reviews. Materials · 2019Article
- Feeling the Force: Measurements of Platelet Contraction and Their Diagnostic Implications.Seminars in thrombosis and hemostasis · 2019Article
- Differential Modulation of Platelet Adhesion and Spreading by Adhesive Ligand Density.Nano letters · 2019Article
- New advances in probing cell-extracellular matrix interactions.Integrative biology : quantitative biosciences from nano to macro · 2017Review
- Assessment of roles for the Rho-specific guanine nucleotide dissociation inhibitor Ly-GDI in platelet function: a spatial systems approach.American journal of physiology. Cell physiology · 2017Article
- Single-platelet nanomechanics measured by high-throughput cytometry.Nature materials · 2017Article
- Topographic Cues Reveal Two Distinct Spreading Mechanisms in Blood Platelets.Scientific reports · 2016Article
- Cell spreading area regulates clathrin-coated pit dynamics on micropatterned substrate.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Article
- Platelet geometry sensing spatially regulates α-granule secretion to enable matrix self-deposition.Blood · 2015Article
- The platelet and the biophysical microenvironment: lessons from cellular mechanics.Thrombosis research · 2014Review
- Systems biology of platelet-vessel wall interactions.Advances in experimental medicine and biology · 2014Review
- Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
To activate clot formation and maintain hemostasis, platelets adhere and spread onto sites of vascular injury. Although this process is well-characterized biochemically, how the physical and spatial cues in the microenvironment affect platelet adhesion and spreading remain unclear. In this study, we applied deep UV photolithography and protein micro/nanostamping to quantitatively investigate and characterize the spatial guidance of platelet spreading at the single cell level and with nanoscale resolution. Platelets adhered to and spread only onto micropatterned collagen or fibrinogen surfaces and followed the microenvironmental geometry with high fidelity and with single micron precision. Using micropatterned lines of different widths, we determined that platelets are able to conform to micropatterned stripes as thin as 0.6 µm and adopt a maximum aspect ratio of 19 on those protein patterns. Interestingly, platelets were also able to span and spread over non-patterned regions of up to 5 µm, a length consistent with that of maximally extended filopodia. This process appears to be mediated by platelet filopodia that are sensitive to spatial cues. Finally, we observed that microenvironmental geometry directly affects platelet biology, such as the spatial organization and distribution of the platelet actin cytoskeleton. Our data demonstrate that platelet spreading is a finely-tuned and spatially-guided process in which spatial cues directly influence the biological aspects of how clot formation is regulated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.