ArticlePloS one2011
Extracellular VirB5 enhances T-DNA transfer from Agrobacterium to the host plant.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 22 citations in OpenAlex.
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- Restriction-ligation-independent production of a TVCV infectious clone and a TVCV-based gene expression vector.Heliyon · 2023Article
- A modified Agrobacterium-mediated transformation for two oomycete pathogens.PLoS pathogens · 2023Article
- Genetic factors governing bacterial virulence and host plant susceptibility duringAdvances in genetics · 2022Review
- Modulation of plant DNA damage response gene expression during Agrobacterium infection.Biochemical and biophysical research communications · 2021Article
- Pathways of DNA Transfer to Plants fromAnnual review of phytopathology · 2019Article
- The Agrobacterium VirE2 effector interacts with multiple members of the Arabidopsis VIP1 protein family.Molecular plant pathology · 2018Article
- Use of chimeric type IV secretion systems to define contributions of outer membrane subassemblies for contact-dependent translocation.Molecular microbiology · 2017Article
- Transcriptional Activation of Virulence Genes of Rhizobium etli.Journal of bacteriology · 2017Article
- Agrobacterium-mediated plant transformation: biology and applications.The arabidopsis book · 2017Article
- A Functional Bacterium-to-Plant DNA Transfer Machinery of Rhizobium etli.PLoS pathogens · 2016Article
- Agrobacterium T-DNA-encoded protein Atu6002 interferes with the host auxin response.Molecular plant pathology · 2014Article
- Article
- Attachment of Agrobacterium to plant surfaces.Frontiers in plant science · 2014Review
- The roles of bacterial and host plant factors in Agrobacterium-mediated genetic transformation.The International journal of developmental biology · 2013Review
- Characterization of VIP1 activity as a transcriptional regulator in vitro and in planta.Scientific reports · 2013Article
- The Agrobacterium VirB/VirD4 T4SS: Mechanism and Architecture Defined Through In Vivo Mutagenesis and Chimeric Systems.Current topics in microbiology and immunologyReview
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
VirB5 is a type 4 secretion system protein of Agrobacterium located on the surface of the bacterial cell. This localization pattern suggests a function for VirB5 which is beyond its known role in biogenesis and/or stabilization of the T-pilus and which may involve early interactions between Agrobacterium and the host cell. Here, we identify VirB5 as the first Agrobacterium virulence protein that can enhance infectivity extracellularly. Specifically, we show that elevating the amounts of the extracellular VirB5--by exogenous addition of the purified protein, its overexpression in the bacterium, or transgenic expression in and secretion out of the host cell--enhances the efficiency the Agrobacterium-mediated T-DNA transfer, as measured by transient expression of genes contained on the transferred T-DNA molecule. Importantly, the exogenous VirB5 enhanced transient T-DNA expression in sugar beet, a major crop recalcitrant to genetic manipulation. Increasing the pool of the extracellular VirB5 did not complement an Agrobacterium virB5 mutant, suggesting a dual function for VirB5: in the bacterium and at the bacterium-host cell interface. Consistent with this idea, VirB5 expressed in the host cell, but not secreted, had no effect on the transformation efficiency. That the increase in T-DNA expression promoted by the exogenous VirB5 was not due to its effects on bacterial growth, virulence gene induction, bacterial attachment to plant tissue, or host cell defense response suggests that VirB5 participates in the early steps of the T-DNA transfer to the plant cell.
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