ArticleTropical medicine and health2011
Multiplex PCR for the concurrent detection and differentiation of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium.
Article in Tropical medicine and health, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Comprehensive epidemiological profiling of poultry-derivedFrontiers in microbiology · 2025Article
- Review
- Association between fluoroquinolone resistance and MRSA genotype in Alexandria, Egypt.Scientific reports · 2021Article
- Regulation of biofilm formation by marT in Salmonella Typhimurium.Molecular biology reports · 2020Article
- Distribution of virulence factors, determinants of antibiotic resistance and molecular fingerprinting of Salmonella species isolated from cattle and beef samples: suggestive evidence of animal-to-meat contamination.Environmental science and pollution research international · 2018Article
- Global transcriptome and mutagenic analyses of the acid tolerance response of Salmonella enterica serovar Typhimurium.Applied and environmental microbiology · 2015Article
- A fine decision tree consisted of CK5/6, IMP3 and TTF1 for cytological diagnosis among reactive mesothelial cells, metastatic adenocarcinoma of lung and non-lung origin in pleural effusion.International journal of clinical and experimental pathology · 2014Article
Corrections and comments
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Authors and funding
12 authors.
Funding
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Abstract
Salmonellosis outbreaks involving typhoid fever and human gastroenteritis are important diseases in tropical countries where hygienic conditions are often not maintained. A rapid and sensitive method to detect Salmonella spp., Salmonella Typhi and Salmonella Typhimurium is needed to improve control and surveillance of typhoid fever and Salmonella gastroenteritis. Our objective was the concurrent detection and differentiation of these food-borne pathogens using a multiplex PCR. We therefore designed and optimized a multiplex PCR using three specific PCR primer pairs for the simultaneous detection of these pathogens. The concentration of each of the primer pairs, magnesium chloride concentration, and primer annealing temperature were optimized before verification of the specificity of the primer pairs. The target genes produced amplicons at 429 bp, 300 bp and 620 bp which were shown to be 100% specific to each target bacterium, Salmonella spp., Salmonella Typhi and Salmonella Typhimurium, respectively.
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