ArticleClinical infectious diseases : an official publication of the Infectious Diseases Society of America2011
Quantitative real-time polymerase chain reaction for enteropathogenic Escherichia coli: a tool for investigation of asymptomatic versus symptomatic infections.
Article in Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 2 syntheses or guidelines pooled it, 82 citations in OpenAlex.
- Systematic Review on the Correlation of Quantitative PCR Cycle Threshold Values of Gastrointestinal Pathogens With Patient Clinical Presentation and Outcomes.Frontiers in medicine · 2021Pooled it
- Global causes of diarrheal disease mortality in children <5 years of age: a systematic review.PloS one · 2013Pooled it
- Oral Phage Therapy of Acute Bacterial Diarrhea With Two Coliphage Preparations: A Randomized Trial in Children From Bangladesh.EBioMedicine · 2016Trial
- Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2015Trial
- Rapid and low-cost detection of six major pediatric enteric pathogens using a closed-tube PCR-invasive reaction assay.Frontiers in cellular and infection microbiology · 2026Article
- Why are so many enteric pathogen infections asymptomatic? Pathogen and gut microbiome characteristics associated with diarrhea symptoms and carriage of diarrheagenicGut microbes · 2023Article
- Colonizing and Virulence Factors in Enterotoxigenic Escherichia coli from Peru.The American journal of tropical medicine and hygiene · 2023Article
- Epidemiology of Enteroaggregative, Enteropathogenic, and Shiga Toxin-Producing Escherichia coli Among Children Aged <5 Years in 3 Countries in Africa, 2015-2018: Vaccine Impact on Diarrhea in Africa (VIDA) Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2023Article
- Clinical Manifestations and Stool Load of Atypical Enteropathogenic Escherichia coli Infections in United States Children and Adults.Gastroenterology · 2022Article
- Enteropathogenic Escherichia coli Infection in Cancer and Immunosuppressed Patients.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Atypical Enteropathogenic Escherichia coli: from Kittens to Humans and Beyond!Infection and immunity · 2021Article
- Quantitative analysis and virulence phenotypes of atypical enteropathogenicGut microbes · 2020Article
- Influence of the intestinal microbiota on disease susceptibility in kittens with experimentally-induced carriage of atypical enteropathogenic Escherichia coli.Veterinary microbiology · 2019Article
- Virulence-Related Genes and Coenteropathogens Associated with Clinical Outcomes of EnteropathogenicJournal of clinical microbiology · 2019Article
- Association between semi-quantitative microbial load and respiratory symptoms among Thai military recruits: a prospective cohort study.BMC infectious diseases · 2018Article
- Association of Atypical Enteropathogenic Escherichia coli with Diarrhea and Related Mortality in Kittens.Journal of clinical microbiology · 2017Article
- Higher atypical enteropathogenic Escherichia coli (a-EPEC) bacterial loads in children with diarrhea are associated with PCR detection of the EHEC factor for adherence 1/lymphocyte inhibitory factor A (efa1/lifa) gene.Annals of clinical microbiology and antimicrobials · 2017Observational
- Use of quantitative molecular diagnostic methods to identify causes of diarrhoea in children: a reanalysis of the GEMS case-control study.Lancet (London, England) · 2016Article
- pic gene of enteroaggregative Escherichia coli and its association with diarrhea in Peruvian children.Pathogens and disease · 2016Article
- Evaluation of a New Device for Simplifying and Standardizing Stool Sample Preparation for Viral Molecular Testing with Limited Hands-On Time.Journal of clinical microbiology · 2016Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 4 countries.
Funding
Abstract
backgroundEnteropathogenic Escherichia coli (EPEC) strains are pediatric pathogens commonly isolated from both healthy and sick children with diarrhea in areas of endemicity. The aim of this study was to compare the bacterial load of EPEC isolated from stool samples from children with and without diarrhea to determine whether bacterial load might be a useful tool for further study of this phenomenon.
methodsEPEC was detected by polymerase chain reaction (PCR) of colonies isolated on MacConkey plates from 53 diarrheal and 90 healthy children aged <2 years. DNA was isolated from stool samples by cetyltrimethylammonium bromide extraction. To standardize quantification by quantitative real-time PCR (qRT-PCR), the correlation between fluorescence threshold cycle and copy number of the intimin gene of EPEC E2348/69 was determined.
resultsThe detection limit of qRT-PCR was 5 bacteria/mg stool. The geometric mean load in diarrhea was 299 bacteria/mg (95% confidence interval [CI], 77-1164 bacteria/mg), compared with 29 bacteria/mg (95% CI, 10-87 bacteria/mg) in control subjects (P = .016). Bacterial load was significantly higher in children with diarrhea than in control subjects among children <12 months of age (178 vs 5 bacteria/mg; P = .006) and among children with EPEC as the sole pathogen (463 vs 24 bacteria/mg; P = .006).
conclusionsEPEC load measured by qRT-PCR is higher in diarrheal than in healthy children. qRT-PCR may be useful to study the relationship between disease and colonization in settings of endemicity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.