Trial reportAmerican journal of physiology. Endocrinology and metabolism2012
Insulin resistance after a 72-h fast is associated with impaired AS160 phosphorylation and accumulation of lipid and glycogen in human skeletal muscle.
Trial report in American journal of physiology. Endocrinology and metabolism, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 72 citations in OpenAlex.
- Fasting-induced glucose intolerance is not impacted by the addition of daily exercise: A randomized crossover study.The Journal of physiology · 2026Trial
- Trial
- Redundancy in regulation of lipid accumulation in skeletal muscle during prolonged fasting in obese men.Physiological reports · 2019Trial
- Trial
- Prolonged fasting-induced metabolic signatures in human skeletal muscle of lean and obese men.PloS one · 2018Trial
- Trial
- Trial
- Protein Ingestion Induces Muscle Insulin Resistance Independent of Leucine-Mediated mTOR Activation.Diabetes · 2015Trial
- Fasting increases human skeletal muscle net phenylalanine release and this is associated with decreased mTOR signaling.PloS one · 2014Trial
- Article
- Systemic metabolic, hormonal, and glycomic remodeling during a 72-hour fast in healthy adults: a pilot study.Croatian medical journal · 2026Article
- The Impact of Breakfast Consumption or Omission on Exercise Performance and Adaptations: A Narrative Review.Nutrients · 2025Review
- Effects of seven days' fasting on physical performance and metabolic adaptation during exercise in humans.Nature communications · 2025Article
- Skeletal muscle from TBC1D4 p.Arg684Ter variant carriers is severely insulin resistant but exhibits normal metabolic responses during exercise.Nature metabolism · 2024Article
- Effects of Fasting on Metabolic Hormones and Functions: A Narrative Review.Juntendo Iji zasshi = Juntendo medical journal · 2024Review
- Short-term fasting lowers glucagon levels under euglycemic and hypoglycemic conditions in healthy humans.JCI insight · 2023Article
- Impact of acute stress on murine metabolomics and metabolic flux.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Roles of Insect Oenocytes in Physiology and Their Relevance to Human Metabolic Diseases.Frontiers in insect science · 2022Review
- Metabolic effects of alternate-day fasting in males with obesity with or without type 2 diabetes.Frontiers in physiology · 2022Article
- Increasing whole-body energetic stress does not augment fasting-induced changes in human skeletal muscle.Pflugers Archiv : European journal of physiology · 2021Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
Abstract
During fasting, human skeletal muscle depends on lipid oxidation for its energy substrate metabolism. This is associated with the development of insulin resistance and a subsequent reduction of insulin-stimulated glucose uptake. The underlying mechanisms controlling insulin action on skeletal muscle under these conditions are unresolved. In a randomized design, we investigated eight healthy subjects after a 72-h fast compared with a 10-h overnight fast. Insulin action on skeletal muscle was assessed by a hyperinsulinemic euglycemic clamp and by determining insulin signaling to glucose transport. In addition, substrate oxidation, skeletal muscle lipid content, regulation of glycogen synthesis, and AMPK signaling were assessed. Skeletal muscle insulin sensitivity was reduced profoundly in response to a 72-h fast and substrate oxidation shifted to predominantly lipid oxidation. This was associated with accumulation of both lipid and glycogen in skeletal muscle. Intracellular insulin signaling to glucose transport was impaired by regulation of phosphorylation at specific sites on AS160 but not TBC1D1, both key regulators of glucose uptake. In contrast, fasting did not impact phosphorylation of AMPK or insulin regulation of Akt, both of which are established upstream kinases of AS160. These findings show that insulin resistance in muscles from healthy individuals is associated with suppression of site-specific phosphorylation of AS160, without Akt or AMPK being affected. This impairment of AS160 phosphorylation, in combination with glycogen accumulation and increased intramuscular lipid content, may provide the underlying mechanisms for resistance to insulin in skeletal muscle after a prolonged fast.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.