ArticleRNA (New York, N.Y.)2011
Cotranscriptional effect of a premature termination codon revealed by live-cell imaging.
Article in RNA (New York, N.Y.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 49 citations in OpenAlex.
- Real-time imaging of transcriptional feedback in nonsense-mediated mRNA decay.Science advances · 2026Article
- Prevalence of and gene regulatory constraints on transcriptional adaptation in single cells.Genome biology · 2024Article
- A Quality Control Mechanism of Splice Site Selection Abrogated under Stress and in Cancer.Cancers · 2022Review
- Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A novel role for nucleolin in splice site selection.RNA biology · 2022Article
- Nonsense-Mediated RNA Decay Factor UPF1 Is Critical for Posttranscriptional and Translational Gene Regulation in Arabidopsis.The Plant cell · 2020Article
- RNA Exosomes and Their Cofactors.Methods in molecular biology (Clifton, N.J.) · 2020Review
- Article
- The RNA surveillance proteins UPF1, UPF2 and SMG6 affect HIV-1 reactivation at a post-transcriptional level.Retrovirology · 2018Article
- mRNA quantification using single-molecule FISH in Drosophila embryos.Nature protocols · 2017Article
- HIV-1 Recruits UPF1 but Excludes UPF2 to Promote Nucleocytoplasmic Export of the Genomic RNA.Biomolecules · 2015Article
- Heat shock activates splicing at latent alternative 5' splice sites in nematodes.Nucleus (Austin, Tex.) · 2015Article
- The Supraspliceosome - A Multi-Task Machine for Regulated Pre-mRNA Processing in the Cell Nucleus.Computational and structural biotechnology journal · 2014Review
- Nonsense-mediated decay factors are involved in the regulation of selenoprotein mRNA levels during selenium deficiency.RNA (New York, N.Y.) · 2014Article
- Single molecule fluorescence approaches shed light on intracellular RNAs.Chemical reviews · 2014Review
- In vivo determination of direct targets of the nonsense-mediated decay pathway in Drosophila.G3 (Bethesda, Md.) · 2014Article
- mRNA quality control goes transcriptional.Biochemical Society transactions · 2013Review
- Translation of pre-spliced RNAs in the nuclear compartment generates peptides for the MHC class I pathway.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Comparison of EJC-enhanced and EJC-independent NMD in human cells reveals two partially redundant degradation pathways.RNA (New York, N.Y.) · 2013Article
- Ribosome profiling reveals resemblance between long non-coding RNAs and 5' leaders of coding RNAs.Development (Cambridge, England) · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Aberrant mRNAs with premature translation termination codons (PTCs) are recognized and eliminated by the nonsense-mediated mRNA decay (NMD) pathway in eukaryotes. We employed a novel live-cell imaging approach to investigate the kinetics of mRNA synthesis and release at the transcription site of PTC-containing (PTC+) and PTC-free (PTC-) immunoglobulin-μ reporter genes. Fluorescence recovery after photobleaching (FRAP) and photoconversion analyses revealed that PTC+ transcripts are specifically retained at the transcription site. Remarkably, the retained PTC+ transcripts are mainly unspliced, and this RNA retention is dependent upon two important NMD factors, UPF1 and SMG6, since their depletion led to the release of the PTC+ transcripts. Finally, ChIP analysis showed a physical association of UPF1 and SMG6 with both the PTC+ and the PTC- reporter genes in vivo. Collectively, our data support a mechanism for regulation of PTC+ transcripts at the transcription site.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.