ArticleThe EMBO journal2012
Myosin-5, kinesin-1 and myosin-17 cooperate in secretion of fungal chitin synthase.
Article in The EMBO journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 117 citations in OpenAlex.
- Cell wall-forming chitin synthases in a chytrid fungus.Npj fungal science · 2026Article
- Revisiting fungal polarized growth: from Spitzenkörper to crescent accumulation of secretory vesicles.iScience · 2025Article
- Fungal Chitin Synthases: Structure, Function, and Regulation.Journal of fungi (Basel, Switzerland) · 2025Review
- Electric field promoted odontogenic differentiation of stem cells from apical papilla by remodelling cytoskeleton.International endodontic journal · 2025Article
- The RhoB p.S73F mutation leads to cerebral palsy through dysregulation of lipid homeostasis.EMBO molecular medicine · 2024Article
- Actin network evolution as a key driver of eukaryotic diversification.Journal of cell science · 2024Review
- Myo5B plays a significant role in the hyphal growth and virulence of the human pathogenic fungusMicrobiology (Reading, England) · 2024Article
- Transcriptional analysis of the dimorphic fungus Umbilicaria muehlenbergii reveals the molecular mechanism of phenotypic transition.World journal of microbiology & biotechnology · 2023Article
- Kinesin-1 autoinhibition facilitates the initiation of dynein cargo transport.The Journal of cell biology · 2023Article
- Chitin Biosynthesis inJournal of fungi (Basel, Switzerland) · 2023Review
- Chitin Synthesis in Yeast: A Matter of Trafficking.International journal of molecular sciences · 2022Review
- Differential venom gland gene expression analysis of juvenile and adult scorpions Androctonus crassicauda.BMC genomics · 2022Article
- The MIT domain of chitin synthase 1 from the oomyceteBiochemistry and biophysics reports · 2022Article
- A Velvet Transcription Factor Specifically Activates Mating through a Novel Mating-Responsive Protein in the Human Fungal Pathogen Cryptococcus deneoformans.Microbiology spectrum · 2022Article
- Myosin-II proteins are involved in the growth, morphogenesis, and virulence of the human pathogenic fungusFrontiers in cellular and infection microbiology · 2022Article
- NADPH Oxidases Play a Role in PathogenicityFrontiers in cellular and infection microbiology · 2022Article
- Genomic Signatures Supporting the Symbiosis and Formation of Chitinous Tube in the Deep-Sea Tubeworm Paraescarpia echinospica.Molecular biology and evolution · 2021Article
- Class V chitin synthase and β(1,3)-glucan synthase co-travel in the same vesicle in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2020Article
- Chitin and chitosan remodeling defines vegetative development and Trichoderma biocontrol.PLoS pathogens · 2020Article
- Off the wall: The rhyme and reason ofCell surface (Amsterdam, Netherlands) · 2019Review
5 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Plant infection by pathogenic fungi requires polarized secretion of enzymes, but little is known about the delivery pathways. Here, we investigate the secretion of cell wall-forming chitin synthases (CHSs) in the corn pathogen Ustilago maydis. We show that peripheral filamentous actin (F-actin) and central microtubules (MTs) form independent tracks for CHSs delivery and both cooperate in cell morphogenesis. The enzyme Mcs1, a CHS that contains a myosin-17 motor domain, is travelling along both MTs and F-actin. This transport is independent of kinesin-3, but mediated by kinesin-1 and myosin-5. Arriving vesicles pause beneath the plasma membrane, but only ~15% of them get exocytosed and the majority is returned to the cell centre by the motor dynein. Successful exocytosis at the cell tip and, to a lesser extent at the lateral parts of the cell requires the motor domain of Mcs1, which captures and tethers the vesicles prior to secretion. Consistently, Mcs1-bound vesicles transiently bind F-actin but show no motility in vitro. Thus, kinesin-1, myosin-5 and dynein mediate bi-directional motility, whereas myosin-17 introduces a symmetry break that allows polarized secretion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.