ArticleThe Journal of biological chemistry2011
Ultrahigh resolution and full-length pilin structures with insights for filament assembly, pathogenic functions, and vaccine potential.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Pilin regions that select for the small RNA phages inJournal of virology · 2025Article
- Type IV pili ofEcoSal Plus · 2024Review
- CryoEM reveals the structure of an archaeal pilus involved in twitching motility.Nature communications · 2024Article
- Understanding a Core Pilin of the Type IVa Pili ofJournal of microbiology and biotechnology · 2024Article
- Tad and toxin-coregulated pilus structures reveal unexpected diversity in bacterial type IV pili.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Disorder and amino acid composition in proteins: their potential role in the adaptation of extracellular pilins to the acidic media, where Acidithiobacillus thiooxidans grows.Extremophiles : life under extreme conditions · 2023Article
- More than a feeling: microscopy approaches to understanding surface-sensing mechanisms.Journal of bacteriology · 2020Review
- The structures of two archaeal type IV pili illuminate evolutionary relationships.Nature communications · 2020Article
- A practical overview of molecular replacement: Clostridioides difficile PilA1, a difficult case study.Acta crystallographica. Section D, Structural biology · 2020Article
- An extensively glycosylated archaeal pilus survives extreme conditions.Nature microbiology · 2019Article
- Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus.Structure (London, England : 1993) · 2019Article
- Dynamics of a type 2 secretion system pseudopilus unraveled by complementary approaches.Journal of biomolecular NMR · 2019Article
- Type IV pili: dynamics, biophysics and functional consequences.Nature reviews. Microbiology · 2019Review
- The Dynamic Structures of the Type IV Pilus.Microbiology spectrum · 2019Review
- The structure of PilA fromThe Journal of biological chemistry · 2019Article
- DNA Uptake by Type IV Filaments.Frontiers in molecular biosciences · 2019Review
- Sequence analysis and confirmation of the type IV pili-associated proteins PilY1, PilW and PilV in Acidithiobacillus thiooxidans.PloS one · 2019Article
- A Pilin Region Affecting Host Range of theFrontiers in microbiology · 2018Article
- Diversity and Evolution of Myxobacterial Type IV Pilus Systems.Frontiers in microbiology · 2018Article
- Cryoelectron Microscopy Reconstructions of the Pseudomonas aeruginosa and Neisseria gonorrhoeae Type IV Pili at Sub-nanometer Resolution.Structure (London, England : 1993) · 2017Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Pilin proteins assemble into Type IV pili (T4P), surface-displayed bacterial filaments with virulence functions including motility, attachment, transformation, immune escape, and colony formation. However, challenges in crystallizing full-length fiber-forming and membrane protein pilins leave unanswered questions regarding pilin structures, assembly, functions, and vaccine potential. Here we report pilin structures of full-length DnFimA from the sheep pathogen Dichelobacter nodosus and FtPilE from the human pathogen Francisella tularensis at 2.3 and 1 Å resolution, respectively. The DnFimA structure reveals an extended kinked N-terminal α-helix, an unusual centrally located disulfide, conserved subdomains, and assembled epitopes informing serogroup vaccines. An interaction between the conserved Glu-5 carboxyl oxygen and the N-terminal amine of an adjacent subunit in the crystallographic dimer is consistent with the hypothesis of a salt bridge between these groups driving T4P assembly. The FtPilE structure identifies an authentic Type IV pilin and provides a framework for understanding the role of T4P in F. tularensis virulence. Combined results define a unified pilin architecture, specialized subdomain roles in pilus assembly and function, and potential therapeutic targets.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.