ArticleNature communications2011
The NMR structure of stomagen reveals the basis of stomatal density regulation by plant peptide hormones.
Article in Nature communications, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 125 citations in OpenAlex.
- Plant peptide hormones: three decades of discovery and emerging paradigms.Plant physiology · 2026Review
- Plant subtilases as key initiators of peptide ligand-receptor signaling.The Plant journal : for cell and molecular biology · 2026Review
- From roots to reproduction: the multifaceted roles of rapid alkalinization factor and epidermal patterning factor peptides in plants.Journal of experimental botany · 2025Review
- Necrosis-Suppressing Effector Protein ChEC88 Adopts a Novel Structural Motif Conserved Among Genus-Spanning Hemibiotrophic Phytopathogens.Plants (Basel, Switzerland) · 2025Article
- Guardians of Water and Gas Exchange: Adaptive Dynamics of Stomatal Development and Patterning.Plants (Basel, Switzerland) · 2025Review
- Comprehensive identification of cotton EPF/EPFL receptors and functional characterization of the GhEPFL1-1-GhER1 module in drought tolerance.BMC plant biology · 2025Article
- Peptide hormones in plants.Molecular horticulture · 2025Review
- Reducing stomatal density by expression of a synthetic epidermal patterning factor increases leaf intrinsic water use efficiency and reduces plant water use in a C4 crop.Journal of experimental botany · 2024Article
- Comparative Genomics Analysis of the Populus Epidermal Pattern Factor (EPF) Family Revealed Their Regulatory Effects inInternational journal of molecular sciences · 2024Article
- An updated model of shoot apical meristem regulation by ERECTA family and CLAVATA3 signaling pathways in Arabidopsis.Development (Cambridge, England) · 2024Article
- Genome-wide analyses of member identification, expression pattern, and protein-protein interaction of EPF/EPFL gene family in Gossypium.BMC plant biology · 2024Article
- Genome-wide identification and phylogenetic and expression pattern analyses of EPF/EPFL family genes in the Rye (Secale cereale L.).BMC genomics · 2024Article
- Genome-wide identification and expression analysis ofFrontiers in genetics · 2024Article
- Genome-Wide Identification and Analysis of the EPF Gene Family inPlants (Basel, Switzerland) · 2023Article
- Paralog editing tunes rice stomatal density to maintain photosynthesis and improve drought tolerance.Plant physiology · 2023Article
- Molecular Mechanisms of Regulation of Root Development by Plant Peptides.Plants (Basel, Switzerland) · 2023Review
- Chemical synthesis of the EPF-family of plant cysteine-rich proteins and late-stage dye attachment by chemoselective amide-forming ligations.RSC chemical biology · 2022Article
- The evolution of stomatal traits along the trajectory toward C4 photosynthesis.Plant physiology · 2022Article
- Article
- Fungal effector SIB1 of Colletotrichum orbiculare has unique structural features and can suppress plant immunity in Nicotiana benthamiana.The Journal of biological chemistry · 2021Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stomatal development in plants is regulated by defensin-like secretory epidermal patterning factor (EPF) peptide hormones. Only one of these, stomagen, is a positive regulator, whereas EPF1, EPF2, and possibly others are negative regulators. Here we explore the structure-function relationships of EPFs, by integrating NMR and semi-in vitro stomagen experiments. We show that stomagen is composed of a loop and a scaffold containing three disulphide bonds. A mutant composed of the stomagen loop and the EPF2 scaffold positively regulates the stomatal density on Arabidopsis cotyledons. The reciprocal mutant composed of the EPF2 loop and the stomagen scaffold acts negatively. Deletion of the disulphide bond introduces unfolding and inactivity. Our results suggest that the loop confers the functional specificity of EPFs and that the scaffold is structurally required for their activity. This structural decomposition approach to elucidating the functional site could be adapted for the analysis of other cysteine-rich peptide families.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.