ArticleCritical care (London, England)2011
mRNA-based approach to monitor recombinant gamma-interferon restoration of LPS-induced endotoxin tolerance.
Article in Critical care (London, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 2 syntheses or guidelines pooled it, 33 citations in OpenAlex.
- Pooled it
- Pooled it
- Early changes of the kinetics of monocyte trem-1 reflect final outcome in human sepsis.BMC immunology · 2014Trial
- Interferon-gamma as adjunctive immunotherapy for invasive fungal infections: a case series.BMC infectious diseases · 2014Trial
- The landscape of immune dysregulation in pediatric sepsis at a single-cell resolution.Clinical immunology (Orlando, Fla.) · 2024Article
- Staging and Personalized Intervention for Infection and Sepsis.Surgical infections · 2020Review
- The role and function of IκKα/β in monocyte impairment.Scientific reports · 2020Article
- Neuroendocrine regulation of innate lymphoid cells.Immunological reviews · 2018Review
- Immune therapy in sepsis: Are we ready to try again?Journal of the Intensive Care Society · 2018Review
- LTR-retrotransposon transcriptome modulation in response to endotoxin-induced stress in PBMCs.BMC genomics · 2018Article
- Source of Circulating Pentraxin 3 in Septic Shock Patients.Frontiers in immunology · 2018Observational
- Host resistance to endotoxic shock requires the neuroendocrine regulation of group 1 innate lymphoid cells.The Journal of experimental medicine · 2017Article
- Defects in innate and adaptive immunity in patients with sepsis and health care associated infection.Annals of translational medicine · 2017Review
- Article
- Immunopathogenesis of abdominal sepsis.Langenbeck's archives of surgery · 2014Review
- S100A8/A9 mRNA induction in an ex vivo model of endotoxin tolerance: roles of IL-10 and IFNγ.PloS one · 2014Article
- Decreased HLA-DR antigen-associated invariant chain (CD74) mRNA expression predicts mortality after septic shock.Critical care (London, England) · 2013Article
- Sepsis-induced immunosuppression: from cellular dysfunctions to immunotherapy.Nature reviews. Immunology · 2013Review
- Local and systemic innate immune response to secondary human peritonitis.Critical care (London, England) · 2013Article
- Identification of biomarkers of response to IFNg during endotoxin tolerance: application to septic shock.PloS one · 2013Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionIt is now well accepted that sepsis is associated with the development of a pronounced immunosuppressive state, characterized by severe immune alterations (e.g. reduced proliferative capacity, endotoxin tolerance, apoptosis) participating in increased mortality and susceptibility to nosocomial infections. Efforts are currently aimed at restoring a functional immune response in septic patients. Successful therapy depends on the identification of appropriate immunostimulatory drugs and on the development of suitable biomarkers that could be used to stratify patients and to follow response to treatment.
methodsIn this study, we evaluated the ex vivo effect of recombinant interferon gamma (rIFN-γ) in restoring monocyte functionality (endotoxin-induced Tumor Necrosis Factor-α production) in a two-hit model of endotoxin tolerance (ET) with peripheral blood mononuclear cells from healthy volunteers and in whole blood of septic shock patients. Importantly, we used quantitative-reverse transcription polymerase-chain reaction to monitor the effect of rIFN-γ on the expression of seven genes known to participate in ET (TNF-α, IL-10, HLA-DRA, CIITA, IRAK-M, ABIN-3 and LY64).
resultsExpression analysis of those genes confirmed the presence of an immunosuppression state and the ex vivo restoration of immune functions by rIFN-γ. We show for the first time that rIFN-γ is able to bypass, at the mRNA level, the effect of negative regulators of the LPS signalling pathway such as IRAK-M, ABIN-3 and LY64.
conclusionsOverall, mRNA expressions of a panel of genes could represent promising candidates for the ex vivo evaluation of rIFN-γ effect on monocyte functionality. This ex vivo translational research study demonstrates the potential of a mRNA-based approach to successfully monitor drug efficacy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.