ArticleNew biotechnology2012
Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation.
Article in New biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Structural insights into human brachyury DNA recognition and discovery of progressible binders for cancer therapy.Nature communications · 2025Article
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- First Observation of an Acetate Switch in a Methanogenic Autotroph (Microbiology insights · 2020Article
- Circulating Levels of Interferon Regulatory Factor-5 Associates With Subgroups of Systemic Lupus Erythematosus Patients.Frontiers in immunology · 2019Article
- 14-3-3 proteins activate Pseudomonas exotoxins-S and -T by chaperoning a hydrophobic surface.Nature communications · 2018Article
- 1,2,6-Thiadiazinones as Novel Narrow Spectrum Calcium/Calmodulin-Dependent Protein Kinase Kinase 2 (CaMKK2) Inhibitors.Molecules (Basel, Switzerland) · 2018Article
- The N-terminal domain of a tick evasin is critical for chemokine binding and neutralization and confers specific binding activity to other evasins.The Journal of biological chemistry · 2018Article
- Highly selective inhibition of histone demethylases by de novo macrocyclic peptides.Nature communications · 2017Article
- A High Through-put Platform for Recombinant Antibodies to Folded Proteins.Molecular & cellular proteomics : MCP · 2015Article
- Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.Nucleic acids research · 2015Article
- Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins.PloS one · 2015Article
- A unique inhibitor binding site in ERK1/2 is associated with slow binding kinetics.Nature chemical biology · 2014Article
- Several affinity tags commonly used in chromatographic purification.Journal of analytical methods in chemistry · 2013Review
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Authors and funding
13 authors.
Funding
Abstract
The generation of affinity reagents to large numbers of human proteins depends on the ability to express the target proteins as high-quality antigens. The Structural Genomics Consortium (SGC) focuses on the production and structure determination of human proteins. In a 7-year period, the SGC has deposited crystal structures of >800 human protein domains, and has additionally expressed and purified a similar number of protein domains that have not yet been crystallised. The targets include a diversity of protein domains, with an attempt to provide high coverage of protein families. The family approach provides an excellent basis for characterising the selectivity of affinity reagents. We present a summary of the approaches used to generate purified human proteins or protein domains, a test case demonstrating the ability to rapidly generate new proteins, and an optimisation study on the modification of >70 proteins by biotinylation in vivo. These results provide a unique synergy between large-scale structural projects and the recent efforts to produce a wide coverage of affinity reagents to the human proteome.
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Registered trials
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