ArticleThe Journal of cell biology1990
Use of DNA sequence and mutant analyses and antisense oligodeoxynucleotides to examine the molecular basis of nonmuscle myosin light chain kinase autoinhibition, calmodulin recognition, and activity.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 167 citations in OpenAlex.
- Regulation of 130-kDa smooth muscle myosin light chain kinase expression by an intronic CArG element.The Journal of biological chemistry · 2013Article
- An integrated mathematical model of thrombin-, histamine-and VEGF-mediated signalling in endothelial permeability.BMC systems biology · 2011Article
- Myosin light chain kinase in microvascular endothelial barrier function.Cardiovascular research · 2010Review
- Regulation of myosin light chain kinase and telokin expression in smooth muscle tissues.American journal of physiology. Cell physiology · 2006Review
- Rho-kinase-mediated Ca2+-independent contraction in rat embryo fibroblasts.American journal of physiology. Cell physiology · 2004Article
- Phosphorylation of myosin II regulatory light chain is necessary for migration of HeLa cells but not for localization of myosin II at the leading edge.The Biochemical journal · 2003Article
- Myosin phosphatase and myosin phosphorylation in differentiating C2C12 cells.Journal of muscle research and cell motility · 2003Article
- Smooth muscle myosin light chain kinase expression in cardiac and skeletal muscle.American journal of physiology. Cell physiology · 2000Article
- Localization and activity of myosin light chain kinase isoforms during the cell cycle.The Journal of cell biology · 2000Article
- DAP kinase and DAP-3: novel positive mediators of apoptosis.Annals of the rheumatic diseases · 1999Review
- Kinase-related protein (telokin) is phosphorylated by smooth-muscle myosin light-chain kinase and modulates the kinase activity.The Biochemical journal · 1997Article
- Smooth muscle myosin light chain kinase is transiently expressed in skeletal muscle during embryogenesis and muscle regeneration both in vivo and in vitro.Journal of muscle research and cell motility · 1997Article
- Regulation of cell motility by mitogen-activated protein kinase.The Journal of cell biology · 1997Article
- DAP-kinase is a Ca2+/calmodulin-dependent, cytoskeletal-associated protein kinase, with cell death-inducing functions that depend on its catalytic activity.The EMBO journal · 1997Article
- Telokin (kinase-related protein) modulates the oligomeric state of smooth-muscle myosin light-chain kinase and its interaction with myosin filaments.The Biochemical journal · 1997Article
- Analysis of cDNAs encoding Drosophila melanogaster myosin light chain kinase.Journal of muscle research and cell motility · 1997Article
- Myosin light chain kinases.Journal of muscle research and cell motility · 1997Review
- Myosin light chain kinase (MLCK) gene disruption in Dictyostelium: a role for MLCK-A in cytokinesis and evidence for multiple MLCKs.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- The Caenorhabditis elegans gene unc-89, required fpr muscle M-line assembly, encodes a giant modular protein composed of Ig and signal transduction domains.The Journal of cell biology · 1996Article
- Expression of a novel myosin light chain kinase in embryonic tissues and cultured cells.The Journal of biological chemistry · 1995Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
The first primary structure for a nonmuscle myosin light chain kinase (nmMLCK) has been determined by elucidation of the cDNA sequence encoding the protein kinase from chicken embryo fibroblasts, and insight into the molecular mechanism of calmodulin (CaM) recognition and activation has been obtained by the use of site-specific mutagenesis and suppressor mutant analysis. Treatment of chicken and mouse fibroblasts with antisense oligodeoxynucleotides based on the cDNA sequence results in an apparent decrease in MLCK levels, an altered morphology reminiscent of that seen in v-src-transformed cells, and a possible effect on cell proliferation. nmMLCK is distinct from and larger than smooth muscle MLCK (smMLCK), although their extended DNA sequence identity is suggestive of a close genetic relationship not found with skeletal muscle MLCK. The analysis of 20 mutant MLCKs indicates that the autoinhibitory and CaM recognition activities are centered in distinct but functionally coupled amino acid sequences (residues 1,068-1,080 and 1,082-1,101, respectively). Analysis of enzyme chimeras, random mutations, inverted sequences, and point mutations in the 1,082-1,101 region demonstrates its functional importance for CaM recognition but not autoinhibition. In contrast, certain mutations in the 1,068-1,080 region result in a constitutively active MLCK that still binds CaM. These results suggest that CaM/protein kinase complexes use similar structural themes to transduce calcium signals into selective biological responses, demonstrate a direct link between nmMLCK and non-muscle cell function, and provide a firm basis for genetic studies and analyses of how nmMLCK is involved in development and cell proliferation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.