ArticleMolecular and cellular biology2012
Architecture of the yeast RNA polymerase II open complex and regulation of activity by TFIIF.
Article in Molecular and cellular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 51 citations in OpenAlex.
- Decoding Transcription Regulatory Mechanisms Associated withmSystems · 2022Article
- The Role of XPB/Ssl2 dsDNA Translocase Processivity in Transcription Start-site Scanning.Journal of molecular biology · 2021Article
- Review
- The origin and evolution of a distinct mechanism of transcription initiation in yeasts.Genome research · 2021Article
- Dynamics of the RNA polymerase I TFIIF/TFIIE-like subcomplex: a mini-review.Biochemical Society transactions · 2020Review
- In vitro reconstitution of yeast RNA polymerase II transcription initiation with high efficiency.Methods (San Diego, Calif.) · 2019Article
- An RNA polymerase II-associated TFIIF-like complex is indispensable for SL RNA gene transcription in Trypanosoma brucei.Nucleic acids research · 2018Article
- Mechanistic Differences in Transcription Initiation at TATA-Less and TATA-Containing Promoters.Molecular and cellular biology · 2018Article
- TFIIH generates a six-base-pair open complex during RNAP II transcription initiation and start-site scanning.Nature structural & molecular biology · 2017Article
- Super elongation complex contains a TFIIF-related subcomplex.Transcription · 2016Article
- Transcription Start Site Scanning and the Requirement for ATP during Transcription Initiation by RNA Polymerase II.The Journal of biological chemistry · 2016Article
- Article
- Uncoupling Promoter Opening from Start-Site Scanning.Molecular cell · 2015Article
- Core promoter sequence in yeast is a major determinant of expression level.Genome research · 2015Article
- Double-stranded DNA translocase activity of transcription factor TFIIH and the mechanism of RNA polymerase II open complex formation.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Structural basis of transcription initiation by RNA polymerase II.Nature reviews. Molecular cell biology · 2015Review
- Article
- Complete architecture of the archaeal RNA polymerase open complex from single-molecule FRET and NPS.Nature communications · 2015Article
- TFIIB is only ∼9 Å away from the 5'-end of a trimeric RNA primer in a functional RNA polymerase II preinitiation complex.PloS one · 2015Article
- Relationships of RNA polymerase II genetic interactors to transcription start site usage defects and growth in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2014Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
To investigate the function and architecture of the open complex state of RNA polymerase II (Pol II), Saccharomyces cerevisiae minimal open complexes were assembled by using a series of heteroduplex HIS4 promoters, TATA binding protein (TBP), TFIIB, and Pol II. The yeast system demonstrates great flexibility in the position of active open complexes, spanning 30 to 80 bp downstream from TATA, consistent with the transcription start site scanning behavior of yeast Pol II. TFIIF unexpectedly modulates the activity of the open complexes, either repressing or stimulating initiation. The response to TFIIF was dependent on the sequence of the template strand within the single-stranded bubble. Mutations in the TFIIB reader and linker region, which were inactive on duplex DNA, were suppressed by the heteroduplex templates, showing that a major function of the TFIIB reader and linker is in the initiation or stabilization of single-stranded DNA. Probing of the architecture of the minimal open complexes with TFIIB-FeBABE [TFIIB-p-bromoacetamidobenzyl-EDTA-iron(III)] derivatives showed that the TFIIB core domain is surprisingly positioned away from Pol II, and the addition of TFIIF repositions the TFIIB core domain to the Pol II wall domain. Together, our results show an unexpected architecture of minimal open complexes and the regulation of activity by TFIIF and the TFIIB core domain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.