ArticleThe Journal of physiology2011
Biophysical properties of slow potassium channels in human embryonic stem cell derived cardiomyocytes implicate subunit stoichiometry.
Article in The Journal of physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 47 citations in OpenAlex.
- Graded activation of mutant K41C-KCNE1:KCNQ1 channel complexes by mefenamic acid.Channels (Austin, Tex.) · 2025Article
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- Gene Expression, Morphology, and Electrophysiology During the Dynamic Development of Human Induced Pluripotent Stem Cell-Derived Atrial- and Ventricular-Like Cardiomyocytes.Biologics : targets & therapy · 2024Article
- Modulation of IJournal of cellular physiology · 2022Article
- MicroRNA-365 regulates human cardiac action potential duration.Nature communications · 2022Article
- Pharmacological rescue of specific long QT variants of KCNQ1/KCNE1 channels.Frontiers in physiology · 2022Article
- Progress and Challenges of Amniotic Fluid Derived Stem Cells in Therapy of Ischemic Heart Disease.International journal of molecular sciences · 2020Review
- Insights into Cardiac IKs (KCNQ1/KCNE1) Channels Regulation.International journal of molecular sciences · 2020Review
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- Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium.BMC biology · 2019Article
- Contribution of potassium channels to action potential repolarization of human embryonic stem cell-derived cardiomyocytes.British journal of pharmacology · 2019Article
- Article
- Gating modulation of the KCNQ1 channel by KCNE proteins studied by voltage-clamp fluorometry.Biophysics and physicobiology · 2019Review
- Transcripts of Kv7.1 and MinK channels and slow delayed rectifier KPflugers Archiv : European journal of physiology · 2018Article
- The IBiophysical journal · 2018Article
- SUMOylation determines the voltage required to activate cardiacProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Human iPSC-Derived Cardiomyocytes for Investigation of Disease Mechanisms and Therapeutic Strategies in Inherited Arrhythmia Syndromes: Strengths and Limitations.Cardiovascular drugs and therapy · 2017Review
- Modeling Inherited Arrhythmia Disorders Using Induced Pluripotent Stem Cell-Derived Cardiomyocytes.Circulation journal : official journal of the Japanese Circulation Society · 2016Review
- Maturation of pluripotent stem cell derived cardiomyocytes: The new challenge.Global cardiology science & practice · 2016Review
- Electrophysiological properties and calcium handling of embryonic stem cell-derived cardiomyocytes.Integrative medicine research · 2016Review
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Human embryonic stem cells (hESCs) are an important cellular model for studying ion channel function in the context of a human cardiac cell and will provide a wealth of information about both heritable arrhythmias and acquired electrophysiological disorders. However, detailed electrophysiological characterization of the important cardiac ion channels has been so far overlooked. Because mutations in the gene for the I(Ks) α subunit, KCNQ1, constitute the majority of long QT syndrome (LQT-1) cases, we have carried out a detailed biophysical analysis of this channel expressed in hESCs to establish baseline I(Ks) channel biophysical properties in cardiac myocytes derived from hESCs (hESC-CMs). I(Ks) channels are heteromultimeric proteins consisting of four identical α-subunits (KCNQ1) assembled with auxiliary β-subunits (KCNE1). We found that the half-maximal I(Ks) activation voltage in hESC-CMs and in myocytes derived from human induced pluripotent stems cells (hiPSC-CMs) falls between that of KCNQ1 channels expressed alone and with full complement of KCNE1, the major KCNE subunit expressed in hESC-CMs as shown by qPCR analysis. Overexpression of KCNE1 by transfection of hESC-CMs markedly shifted and slowed native I(Ks) activation implying assembly of additional KCNE1 subunits with endogenous channels. Our results in hESC-CMs, which indicate an I(Ks) subunit stoichiometry that can be altered by variable KCNE1 expression, suggest the possibility for variable I(Ks) function in the developing heart, in different tissues in the heart, and in disease. This establishes a new baseline for I(Ks) channel properties in myocytes derived from pluripotent stem cells and will guide future studies in patient-specific hiPSCs.
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