Evidence map›Paper›PMID 22025511›Full record

ArticleInfection and immunity2012

Enterobacterial common antigen mutants of Salmonella enterica serovar Typhimurium establish a persistent infection and provide protection against subsequent lethal challenge.

Jeremy J Gilbreath, Jennifer Colvocoresses Dodds, Paul D Rick, Mark J Soloski, D Scott Merrell, Eleanor S Metcalf

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Infection and immunity, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 30 citations in OpenAlex.

  1. Article
  2. Functional loss ofFrontiers in microbiology · 2025
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  7. Frontiers in microbiology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Jeremy J GilbreathDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.
Jennifer Colvocoresses Dodds
Paul D Rick
Mark J Soloski
D Scott Merrell
Eleanor S Metcalf
Uniformed Services University of the Health Sciences · USJohns Hopkins University · US

Funding

T CELL RESPONSES TO INTRACELLULAR BACTERIAL PATHOGENSR01AI042287 · NIAID · JOHNS HOPKINS UNIVERSITY · PI SOLOSKI, MARK J · 1998 to 2008
$2.3M
Regulatory Networks of Helicobacter pyloriR01AI065529 · NIAID · HENRY M. JACKSON FDN FOR THE ADV MIL/MED · PI MERRELL, D. SCOTT · 2007 to 2011
$1.9M
Regulatory Mechanisms of Helicobacter pyloriR56AI065529 · NIAID · HENRY M. JACKSON FDN FOR THE ADV MIL/MED · PI MERRELL, D. SCOTT · 2013 to 2013
$357k
T CELL RESPONSES TO INTRACELLULAR BACTERIAL PATHOGENSR21AI042287 · NIAID · JOHNS HOPKINS UNIVERSITY · PI SOLOSKI, MARK J · 2003 to 2003
$357k
NIAID NIH HHS AI065529NIAID NIH HHS AI42287NIAID NIH HHS R01 AI042287NIAID NIH HHS R01 AI065529NIAID NIH HHS R21 AI042287NIAID NIH HHS R56 AI065529
6 · The paper itself

Abstract

Infection with Salmonella spp. is a significant source of disease globally. A substantial proportion of these infections are caused by Salmonella enterica serovar Typhimurium. Here, we characterize the role of the enterobacterial common antigen (ECA), a surface glycolipid ubiquitous among enteric bacteria, in S. Typhimurium pathogenesis. Construction of a defined mutation in the UDP-N-acetylglucosamine-1-phosphate transferase gene, wecA, in two clinically relevant strains of S. Typhimurium, TML and SL1344, resulted in strains that were unable to produce ECA. Loss of ECA did not affect the gross cell surface ultrastructure, production of lipopolysaccharide (LPS), flagella, or motility. However, the wecA mutant strains were attenuated in both oral and intraperitoneal mouse models of infection (P<0.001 for both routes of infection; log rank test), and virulence could be restored by complementation of the wecA gene in trans. Despite the avirulence of the ECA-deficient strains, the wecA mutant strains were able to persistently colonize systemic sites (spleen and liver) at moderate levels for up to 70 days postinfection. Moreover, immunization with the wecA mutant strains provided protection against a subsequent lethal oral or intraperitoneal challenge with wild-type S. Typhimurium. Thus, wecA mutant (ECA-negative) strains of Salmonella may be useful as live attenuated vaccine strains or as vehicles for heterologous antigen expression.

Indexed as

Gene DeletionAnimalsAntigens, BacterialBacterial ProteinsFemaleGenetic Complementation TestLiverMiceMice, Inbred C57BLMutant ProteinsSalmonella Infections, AnimalSalmonella typhimuriumSpleenSurvival AnalysisTransferases (Other Substituted Phosphate Groups)Virulence FactorsAntigens, BacterialBacterial Proteinsenterobacterial common antigenMutant ProteinsTransferases (Other Substituted Phosphate Groups)UDP-GlcNAc-undecaprenyl phosphate N-acetylglucosaminyl 1-phosphate transferaseVirulence Factors

Identifiers

PMID22025511
PMCPMC3255661
OpenAlexW2034391918

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.