ArticleAntimicrobial agents and chemotherapy2012
Genetic environment and stability of cfr in methicillin-resistant Staphylococcus aureus CM05.
Article in Antimicrobial agents and chemotherapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 65 citations in OpenAlex.
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- Mobile Oxazolidinone Resistance Genes in Gram-Positive and Gram-Negative Bacteria.Clinical microbiology reviews · 2021Article
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- Using genomics to understand meticillin- and vancomycin-resistantMicrobial genomics · 2020Review
- Expansion of highly stable blaBMC research notes · 2017Article
- Pleuromutilins: Potent Drugs for Resistant Bugs-Mode of Action and Resistance.Cold Spring Harbor perspectives in medicine · 2017Review
- Transcriptional analysis ofAntimicrobial resistance and infection control · 2017Article
- Lincosamides, Streptogramins, Phenicols, and Pleuromutilins: Mode of Action and Mechanisms of Resistance.Cold Spring Harbor perspectives in medicine · 2016Review
- Toxin-Antitoxin Systems in Clinical Pathogens.Toxins · 2016Review
- Occurrence of co-existing bla VIM-2 and bla NDM-1 in clinical isolates of Pseudomonas aeruginosa from India.Annals of clinical microbiology and antimicrobials · 2016Article
- Toxin-Antitoxin Systems of Staphylococcus aureus.Toxins · 2016Review
- First Report of cfr-Carrying Plasmids in the Pandemic Sequence Type 22 Methicillin-Resistant Staphylococcus aureus Staphylococcal Cassette Chromosome mec Type IV Clone.Antimicrobial agents and chemotherapy · 2016Article
- In vitro activity of tedizolid against linezolid-resistant staphylococci and enterococci.Diagnostic microbiology and infectious disease · 2016Article
- Tedizolid Phosphate: a Next-Generation Oxazolidinone.Infectious diseases and therapy · 2015Article
- Article
- Anti-alpha-toxin monoclonal antibody and antibiotic combination therapy improves disease outcome and accelerates healing in a Staphylococcus aureus dermonecrosis model.Antimicrobial agents and chemotherapy · 2015Article
- Linezolid-resistant Staphylococcus aureus strain 1128105, the first known clinical isolate possessing the cfr multidrug resistance gene.Antimicrobial agents and chemotherapy · 2014Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cfr methyltransferase confers resistance to many 50S ribosomal subunit-targeted antibiotics, including linezolid (LZD), via methylation of the 23S rRNA base A2503 in the peptidyl transferase center. Methicillin-resistant Staphylococcus aureus strain CM05 is the first clinical isolate documented to carry cfr. While cfr is typically plasmid borne, in CM05 it is located on the chromosome and is coexpressed with ermB as part of the mlr operon. Here we evaluated the chromosomal locus, association with mobile genetic elements, and stability of the cfr insertion region in CM05. The cfr-containing mlr operon is located within a 15.5-kb plasmid-like insertion into 23S rRNA allele 4. The region surrounding the cfr gene has a high degree of sequence similarity to the broad-host-range toxin/antitoxin multidrug resistance plasmid pSM19035, including a second ermB gene downstream of the mlr locus and istAS-istBS. Analysis of several individual CM05 colonies revealed two distinct populations for which LZD MICs were either 8 or 2 μg/ml. In the LZD(s) colonies (designated CM05Δ), a recombination event involving the two ermB genes had occurred, resulting in the deletion of cfr and the 3' flanking region (cfr-istAS-istBS-ermB). The fitness advantage of CM05Δ over CM05 (though not likely due to the cfr deletion itself) results in the predominance of CM05Δ in the absence of selective pressure. Minicircles resulting from the ermB recombination event and the novel association of cfr with the pSM19035 plasmid system support the potential for the continued dissemination of cfr.
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