ArticleAnesthesiology2011
A gain-of-function mutation in adenylate cyclase confers isoflurane resistance in Caenorhabditis elegans.
Article in Anesthesiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Induction and regulation of reversible suspended animation in C. elegans.Nature communications · 2026Article
- Induction and regulation of a reversible form of suspended animation inbioRxiv : the preprint server for biology · 2026Article
- Current and Developing Approaches for Facilitating Emergence from General Anesthesia.Anesthesiology · 2025Review
- Functional Insights into Protein Kinase A (PKA) Signaling fromLife (Basel, Switzerland) · 2022Review
- Dysregulation of Neuronal Gαo Signaling by Graphene Oxide in Nematode Caenorhabditis elegans.Scientific reports · 2019Article
- PDF-1 neuropeptide signaling regulates sexually dimorphic gene expression in shared sensory neurons ofeLife · 2018Article
- Caffeine accelerates recovery from general anesthesia via multiple pathways.Journal of neurophysiology · 2017Article
- Pheromone-sensing neurons regulate peripheral lipid metabolism in Caenorhabditis elegans.PLoS genetics · 2017Article
- Habituation as an adaptive shift in response strategy mediated by neuropeptides.NPJ science of learning · 2017Article
- Behavioral analysis of the huntingtin-associated protein 1 ortholog trak-1 in Caenorhabditis elegans.Journal of neuroscience research · 2016Article
- Distinct Mechanisms Underlie Quiescence during Two Caenorhabditis elegans Sleep-Like States.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Limitations of the 'ambush hypothesis' at the single-gene scale: what codon biases are to blame?Molecular genetics and genomics : MGG · 2015Article
- Caenorhabditis elegans dnj-14, the orthologue of the DNAJC5 gene mutated in adult onset neuronal ceroid lipofuscinosis, provides a new platform for neuroprotective drug screening and identifies a SIR-2.1-independent action of resveratrol.Human molecular genetics · 2014Article
- Caffeine accelerates recovery from general anesthesia.Journal of neurophysiology · 2014Article
- Serotonin and the neuropeptide PDF initiate and extend opposing behavioral states in C. elegans.Cell · 2013Article
- Induced changes in protein receptors conferring resistance to anesthetics.Current opinion in anaesthesiology · 2012Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundVolatile general anesthetics inhibit neurotransmitter release by a mechanism not fully understood. Genetic evidence in Caenorhabditis elegans has shown that a major mechanism of action of volatile anesthetics acting at clinical concentrations in this animal is presynaptic inhibition of neurotransmission. To define additional components of this presynaptic volatile anesthetic mechanism, C. elegans mutants isolated as phenotypic suppressors of a mutation in syntaxin, an essential component of the neurotransmitter release machinery, were screened for anesthetic sensitivity phenotypes.
methodsSensitivity to isoflurane concentrations was measured in locomotion assays on adult C. elegans. Sensitivity to the acetylcholinesterase inhibitor aldicarb was used as an assay for the global level of C. elegans acetylcholine release. Comparisons of isoflurane sensitivity (measured by the EC₅₀) were made by simultaneous curve-fitting and F test.
resultsAmong the syntaxin suppressor mutants, js127 was the most isoflurane resistant, with an EC₅₀ more than 3-fold that of wild type. Genetic mapping, sequencing, and transformation phenocopy showed that js127 was an allele of acy-1, which encodes an adenylate cyclase expressed throughout the C. elegans nervous system and in muscle. js127 behaved as a gain-of-function mutation in acy-1 and had increased concentrations of cyclic adenosine monophosphate. Testing of single and double mutants along with selective tissue expression of the js127 mutation revealed that acy-1 acts in neurons within a Gαs-PKA-UNC-13-dependent pathway to regulate behavior and isoflurane sensitivity.
conclusionsActivation of neuronal adenylate cyclase antagonizes isoflurane inhibition of locomotion in C. elegans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.