ArticleThe Journal of cell biology2011
Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation.
Article in The Journal of cell biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Review
- Article
- Dbf4-Dependent Kinase: DDK-ated to post-initiation events in DNA replication.Cell cycle (Georgetown, Tex.) · 2021Review
- Human DDK rescues stalled forks and counteracts checkpoint inhibition at unfired origins to complete DNA replication.Molecular cell · 2021Article
- Both a Unique Motif at the C Terminus and an N-Terminal HEAT Repeat Contribute to G-Quadruplex Binding and Origin Regulation by the Rif1 Protein.Molecular and cellular biology · 2019Article
- Dependency of Heterochromatin Domains on Replication Factors.G3 (Bethesda, Md.) · 2018Article
- Mrc1/Claspin: a new role for regulation of origin firing.Current genetics · 2017Review
- Checkpoint-Independent Regulation of Origin Firing by Mrc1 through Interaction with Hsk1 Kinase.Molecular and cellular biology · 2017Article
- Rif1 binds to G quadruplexes and suppresses replication over long distances.Nature structural & molecular biology · 2015Article
- Switch on the engine: how the eukaryotic replicative helicase MCM2-7 becomes activated.Chromosoma · 2015Review
- Helicase activation and establishment of replication forks at chromosomal origins of replication.Cold Spring Harbor perspectives in biology · 2013Review
- Sequential steps in DNA replication are inhibited to ensure reduction of ploidy in meiosis.Molecular biology of the cell · 2013Article
- Rif1 is a global regulator of timing of replication origin firing in fission yeast.Genes & development · 2012Article
- Regulation of DNA replication timing on human chromosome by a cell-type specific DNA binding protein SATB1.PloS one · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cdc7/Hsk1 is a conserved kinase required for initiation of DNA replication that potentially regulates timing and locations of replication origin firing. Here, we show that viability of fission yeast hsk1Δ cells can be restored by loss of mrc1, which is required for maintenance of replication fork integrity, by cds1Δ, or by a checkpoint-deficient mutant of mrc1. In these mutants, normally inactive origins are activated in the presence of hydroxyurea and binding of Cdc45 to MCM is stimulated. mrc1Δ bypasses hsk1Δ more efficiently because of its checkpoint-independent inhibitory functions. Unexpectedly, hsk1Δ is viable at 37°C. More DNA is synthesized, and some dormant origins fire in the presence of hydroxyurea at 37°C. Furthermore, hsk1Δ bypass strains grow poorly at 25°C compared with higher temperatures. Our results show that Hsk1 functions for DNA replication can be bypassed by different genetic backgrounds as well as under varied physiological conditions, providing additional evidence for plasticity of the replication program in eukaryotes.
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