ReviewBiochimica et biophysica acta2012
Subunit III-depleted cytochrome c oxidase provides insight into the process of proton uptake by proteins.
Review in Biochimica et biophysica acta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Mitochondrial complex-1 as a therapeutic target for cardiac diseases.Molecular and cellular biochemistry · 2025Review
- Effects of Microplastic (MP) Exposure at Environmentally Relevant Doses on the Structure, Function, and Transcriptome of the Kidney in Mice.Molecules (Basel, Switzerland) · 2023Article
- Proton transfer reactions: From photochemistry to biochemistry and bioenergetics.BBA advances · 2023Article
- Structure and Mechanism of Respiratory III-IV Supercomplexes in Bioenergetic Membranes.Chemical reviews · 2021Review
- Effect of HBx on inflammation and mitochondrial oxidative stress in mouse hepatocytes.Oncology letters · 2020Article
- Structural changes at the surface of cytochrome c oxidase alter the proton-pumping stoichiometry.Biochimica et biophysica acta. Bioenergetics · 2020Article
- Hypoxia-inducible gene domain 1 proteins in yeast mitochondria protect against proton leak through complex IV.The Journal of biological chemistry · 2019Article
- Mitochondrial electron transport chain, ROS generation and uncoupling (Review).International journal of molecular medicine · 2019Article
- Cytochrome aaBiochemistry · 2018Article
- Voltage and pH sensing by the voltage-gated proton channel, HJournal of the Royal Society, Interface · 2018Review
- Delipidation of cytochrome c oxidase from Rhodobacter sphaeroides destabilizes its quaternary structure.Biochimie · 2016Article
- Control of carotenoid biosynthesis through a heme-based cis-trans isomerase.Nature chemical biology · 2015Article
- Article
- Mitochondrial-nuclear genome interactions in non-alcoholic fatty liver disease in mice.The Biochemical journal · 2014Article
- Role of norEF in denitrification, elucidated by physiological experiments with Rhodobacter sphaeroides.Journal of bacteriology · 2014Article
- Philosophy of voltage-gated proton channels.Journal of the Royal Society, Interface · 2014Review
- Peregrination of the selectivity filter delineates the pore of the human voltage-gated proton channel hHV1.The Journal of general physiology · 2013Article
Corrections and comments
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2 authors.
Funding
Abstract
We review studies of subunit III-depleted cytochrome c oxidase (CcO III (-)) that elucidate the structural basis of steady-state proton uptake from solvent into an internal proton transfer pathway. The removal of subunit III from R. sphaeroides CcO makes proton uptake into the D pathway a rate-determining step, such that measurements of the pH dependence of steady-state O(2) consumption can be used to compare the rate and functional pK(a) of proton uptake by D pathways containing different initial proton acceptors. The removal of subunit III also promotes spontaneous suicide inactivation by CcO, greatly shortening its catalytic lifespan. Because the probability of suicide inactivation is controlled by the rate at which the D pathway delivers protons to the active site, measurements of catalytic lifespan provide a second method to compare the relative efficacy of proton uptake by engineered CcO III (-) forms. These simple experimental systems have been used to explore general questions of proton uptake by proteins, such as the functional value of an initial proton acceptor, whether an initial acceptor must be surface-exposed, which side chains will function as initial proton acceptors and whether multiple acceptors can speed proton uptake.
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