ArticleNano letters2011
Detecting intramolecular conformational dynamics of single molecules in short distance range with subnanometer sensitivity.
Article in Nano letters, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- Overcoming aggregation-induced quenching in DNA-assembled rhodamine dimers.Physical chemistry chemical physics : PCCP · 2025Article
- Labelizer: systematic selection of protein residues for covalent fluorophore labeling.Nature communications · 2025Article
- Linker Molecules Convert Commercial Fluorophores into Tailored Functional Probes during Biolabelling.Angewandte Chemie (International ed. in English) · 2022Article
- Single-Molecule FRET Detection of Sub-Nanometer Distance Changes in the Range below a 3-Nanometer Scale.Biosensors · 2020Article
- Ligand discrimination between active and inactive activation loop conformations of Aurora-A kinase is unmodified by phosphorylation.Chemical science · 2019Article
- The Tiam1 guanine nucleotide exchange factor is auto-inhibited by its pleckstrin homology coiled-coil extension domain.The Journal of biological chemistry · 2017Article
- Dynamic Equilibrium of the Aurora A Kinase Activation Loop Revealed by Single-Molecule Spectroscopy.Angewandte Chemie (International ed. in English) · 2017Article
- DNA-Endonuclease Complex Dynamics by Simultaneous FRET and Fluorophore Intensity in Evanescent Field.Biophysical journal · 2017Article
- PCNA tool belts and polymerase bridges form during translesion synthesis.Nucleic acids research · 2016Article
- Förster resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers.Scientific reports · 2016Article
- The Proliferating Cell Nuclear Antigen (PCNA)-interacting Protein (PIP) Motif of DNA Polymerase η Mediates Its Interaction with the C-terminal Domain of Rev1.The Journal of biological chemistry · 2016Article
- Quantifying the Assembly of Multicomponent Molecular Machines by Single-Molecule Total Internal Reflection Fluorescence Microscopy.Methods in enzymology · 2016Article
- Binding dynamics of a monomeric SSB protein to DNA: a single-molecule multi-process approach.Nucleic acids research · 2015Article
- Single-Molecule Pull-Down FRET to Dissect the Mechanisms of Biomolecular Machines.Methods in enzymology · 2015Article
- Shedding light on protein folding, structural and functional dynamics by single molecule studies.Molecules (Basel, Switzerland) · 2014Review
- Direct correlation of DNA binding and single protein domain motion via dual illumination fluorescence microscopy.Nano letters · 2014Article
- Ultrafast redistribution of E. coli SSB along long single-stranded DNA via intersegment transfer.Journal of molecular biology · 2014Article
- Article
- Role of the primer activation signal in tRNA annealing onto the HIV-1 genome studied by single-molecule FRET microscopy.RNA (New York, N.Y.) · 2013Article
- Direct visualization of the reaction transformation and signal amplification in a DNA molecular machine with total internal reflection fluorescence microscopy.Frontiers in chemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Single molecule detection is useful for characterizing nanoscale objects such as biological macromolecules, nanoparticles and nanodevices with nanometer spatial resolution. Fluorescence resonance energy transfer (FRET) is widely used as a single-molecule assay to monitor intramolecular dynamics in the distance range of 3-8 nm. Here we demonstrate that self-quenching of two rhodamine derivatives can be used to detect small conformational dynamics corresponding to subnanometer distance changes in a FRET-insensitive short-range at the single molecule level. A ParM protein mutant labeled with two rhodamines works as a single molecule adenosine 5'-diphosphate (ADP) sensor that has 20 times brighter fluorescence signal in the ADP bound state than the unbound state. Single molecule time trajectories show discrete transitions between fluorescence on and off states that can be directly ascribed to ADP binding and dissociation events. The conformational changes observed with 20:1 contrast are only 0.5 nm in magnitude and are between crystallographic distances of 1.6 and 2.1 nm, demonstrating exquisite sensitivity to short distance scale changes. The systems also allowed us to gain information on the photophysics of self-quenching induced by rhodamine stacking: (1) photobleaching of either of the two rhodamines eliminates quenching of the other rhodamine fluorophore and (2) photobleaching from the highly quenched, stacked state is only 2-fold slower than from the unstacked state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.