ArticlePloS one2011
Expression of novel opsins and intrinsic light responses in the mammalian retinal ganglion cell line RGC-5. Presence of OPN5 in the rat retina.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Unveiling Glial Heterogeneity in the Inner Retina: Non-Visual Photic Spectrum Responses and Calcium Dynamics in Müller Glial Cells vs. Neurons.International journal of molecular sciences · 2026Article
- Non-visual opsins and their role in circadian photoentrainment.Eye and vision (London, England) · 2026Review
- Direct Stimulation of Gastric Smooth Muscle Cells via GNeurogastroenterology and motility · 2025Article
- Opsin 5 mediates violet light-induced early growth response-1 expression in the mouse retina.Scientific reports · 2023Article
- A Case Study from the Past: "The RGC-5 vs. the 661W Cell Line: Similarities, Differences and Contradictions-Are They Really the Same?"International journal of molecular sciences · 2023Article
- Ocular and extraocular roles of neuropsin in vertebrates.Trends in neurosciences · 2022Review
- Non-visual Opsins and Novel Photo-Detectors in the Vertebrate Inner Retina Mediate Light Responses Within the Blue Spectrum Region.Cellular and molecular neurobiology · 2022Review
- Opsin 5 is a key regulator of ultraviolet radiation-induced melanogenesis in human epidermal melanocytes.The British journal of dermatology · 2021Article
- Article
- Localization of the ultraviolet-sensor Opn5m and its effect on myopia-related gene expression in the late-embryonic chick eye.Biochemistry and biophysics reports · 2019Article
- Expression of Non-visual Opsins Opn3 and Opn5 in the Developing Inner Retinal Cells of Birds. Light-Responses in Müller Glial Cells.Frontiers in cellular neuroscience · 2019Article
- Implication of Melanopsin and Trigeminal Neural Pathways in Blue Light PhotosensitivityFrontiers in neuroscience · 2019Article
- Ocular Clocks: Adapting Mechanisms for Eye Functions and Health.Investigative ophthalmology & visual science · 2018Review
- Peropsin modulates transit of vitamin A from retina to retinal pigment epithelium.The Journal of biological chemistry · 2017Article
- Non-Visual Photopigments Effects of Constant Light-Emitting Diode Light Exposure on the Inner Retina of Wistar Rats.Frontiers in neurology · 2017Article
- Inhibition on Apoptosis Induced by Elevated Hydrostatic Pressure in Retinal Ganglion Cell-5 via Laminin Upregulating β1-integrin/Focal Adhesion Kinase/Protein Kinase B Signaling Pathway.Chinese medical journal · 2016Article
- Light-induced rapid Ca²⁺ response and MAPK phosphorylation in the cells heterologously expressing human OPN5.Scientific reports · 2014Article
- Evolution of mammalian Opn5 as a specialized UV-absorbing pigment by a single amino acid mutation.The Journal of biological chemistry · 2014Article
- Article
- Effects of optineurin siRNA on apoptotic genes and apoptosis in RGC-5 cells.Molecular vision · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The vertebrate retina is known to contain three classes of photoreceptor cells: cones and rods responsible for vision, and intrinsically photoresponsive retinal ganglion cells (RGCs) involved in diverse non-visual functions such as photic entrainment of daily rhythms and pupillary light responses. In this paper we investigated the potential intrinsic photoresponsiveness of the rat RGC line, RGC-5, by testing for the presence of visual and non-visual opsins and assessing expression of the immediate-early gene protein c-Fos and changes in intracellular Ca(2+) mobilization in response to brief light pulses. Cultured RGC-5 cells express a number of photopigment mRNAs such as retinal G protein coupled receptor (RGR), encephalopsin/panopsin (Opn3), neuropsin (Opn5) and cone opsin (Opn1mw) but not melanopsin (Opn4) or rhodopsin. Opn5 immunoreactivity was observed in RGC-5 cells and in the inner retina of rat, mainly localized in the ganglion cell layer (GCL). Furthermore, white light pulses of different intensities and durations elicited changes both in intracellular Ca(2+) levels and in the induction of c-Fos protein in RGC-5 cell cultures. The results demonstrate that RGC-5 cells expressing diverse putative functional photopigments display intrinsic photosensitivity which accounts for the photic induction of c-Fos protein and changes in intracellular Ca(2+) mobilization. The presence of Opn5 in the GCL of the rat retina suggests the existence of a novel type of photoreceptor cell.
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